18种中药对猪大肠杆菌的体外抑菌活性的测定方法比较  

Study on the in vitro Bacteriostatic Test of 17 Kinds of Traditional Chinese Herbs against Swine E.coli

作　　者：王俊丽 张要齐 孙雪峰 张新蕾 陈静 张妍 张玲 

机构地区：[1]河南惠通天下生物工程有限公司,河南新郑451162 [2]河南惠通天下动物药业有限公司,河南郑州450008

出　　处：《安徽农业科学》2012年第26期12947-12948,12951,共3页Journal of Anhui Agricultural Sciences

基　　金：郑州市金水区科技攻关计划项目(金科[2011]31号)

摘　　要：[目的]比较2种体外抑菌试验结果,筛选猪大肠杆菌的抑菌中药,为临床用药及开发中兽药制剂提供依据。[方法]采用水煎法制备18种中药水提物,分别通过试管二倍稀释法和管碟法进行体外抑菌试验。[结果]试管二倍稀释法中18种中药均表现出一定的抑菌效果,但管碟法测定部分中药表现出耐药性。2种测定方法中黄连、大黄抑菌效果最强,对猪大肠杆菌的MIC均为7.82 mg/ml,MBC值也相对较高,抑菌圈直径分别为25.28和22.84 mm,而穿心莲、白头翁、防风等抑菌效果相对较差。[结论]不同中药对猪大肠杆菌的抑菌活性不同,采用不同的测定方法所得试验结果既有一致性又有差异性。[ Objective ] To compare the results of two in vitro antibacterial tests, and to screen out Chinese herbs with strong bacteriostatic activity and provide foundation for clinical medication and developing Chinese herbal medicine preparations. [ Method] The water extracts of 18 kinds of Chinese herbs were prepared by water decoction method and the becteriostatic test in vitro against swine Escherichia coli was determined by tube double dilution method and cup-plate method. [ Result] 18 kinds of traditional Chinese medicine showed a certain inhibitory effect in the tube double dilution method, but part of traditional Chinese medicine showed resistance in the plate method. Rheum officinale and Coptis chinensis had the strongest inhibitory effect in two methods, their MIC for Escherichia coli was both 7.82 mg/ml, and MBC values were relatively higher. The inhibition zone diameter was 25.28 and 22.84 mm. Andrographis paniculata ,Anemone chinensis,Saposhnikovia divaricata, etc. ,bad inhibitory effect. [ Conclusion ] Different herbal medicines show different bacteriostatic effects against swine E. coli, the results of different determination methods show consistency as well as difference.

关 键 词：中药 猪大肠杆菌 试管二倍稀释法 管碟法 体外抑菌试验 

分 类 号：S567[农业科学—中草药栽培]