辣椒单倍体离体诱导及育种应用  

In vitro Haploid Production and Application to Pepper Breeding

作　　者：张正海[1] 毛胜利[1] 王立浩[1] 张宝玺[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京l00081

出　　处：《园艺学报》2012年第9期1715-1726,共12页Acta Horticulturae Sinica

基　　金：‘十二五’农村领域国家科技计划课题(2012AA100200)

摘　　要：概括介绍了辣椒单倍体诱导的重要进展,对影响花药和小孢子培养的材料、预处理、培养基种类、离体雄核发育的细胞学标记及过程,以及单倍体诱导技术在辣椒杂交育种中的应用进行了综述。In this work, we summarize the brief history of in vitro androgenesis in pepper, with reviews of factors affecting haploid induction which containing growing conditions and donor plant genotype, microspore developmental stage, abiotic stresses and culture media and its supplements, cytological studies of in vitro androgenesis, practical application of haploid technique in Capsicum breeding. Finally, we outline the drawbacks of haploid technology for pepper breeding and potential solutions to overcome genotype dependency and high frequency of abnormal microspore-derived embryos in anther and microspore culture.

关 键 词：辣椒 离体雄核发育 单倍体 花药培养 小孢子培养 

分 类 号：S641.3[农业科学—蔬菜学]