缺血后适应在兔急性肠系膜缺血再灌注损伤模型中的作用  

The study of ischemia postconditioning intervention in a rabbit＇s acute mesenteric ischemia- reperfusion injury model

作　　者：杨牟[1] 张居文[1] 陈萍[1] 孙林[1] 车海杰[1] 勇俊[1] 李鲁滨[1] 宋富波[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院血管外科,山东省264000

出　　处：《中华普通外科杂志》2012年第9期740-742,共3页Chinese Journal of General Surgery

基　　金：烟台市科技发展计划项目（2008142-32）

摘　　要：目的观察缺血后适应干预措施能否减轻兔急性肠系膜缺血再灌注损伤。方法将雄性新西兰兔120只随机分为空白对照（Con）组（仅开腹显露SMA）、缺血再灌注（I／R）组[肠系上动脉（superior mesenteric artery，SMA）缺血30min后持续灌注120min]、缺血后适应1（IpostC1）组（SMA缺血30min后行3个循环的灌注30s／，阻断308，再持续灌注117min）、缺血后适应2（IpostC2）组（SMA缺血30min后行3个循环的灌注60s／阻断60s，再持续灌注114min），每组30只。干预后采集各组兔处理后下腔静脉血及部分肠道组织标本，试剂盒测定血清及肠道组织内丙二醛（MDA）、髓过氧化酶（MPO）水平，HE染色，Chiu6级评分法观察肠黏膜损伤情况。结果与对照组比较，I／R组及IpostC1组、IpostC2组血清及肠道组织中MDA、MPO水平明显增加（P〈0．01），肠黏膜损伤评分明显升高（P〈0．01）。与I／R组相比，IpostC1组血清和肠道组织中MDA、MPO水平及肠黏膜损伤评分明显降低（P〈0．01），而IpostC2组血清和肠道组织中MDA、MPO水平及肠黏膜损伤评分无明显降低（P〉0．05）。结论缺血后适应可明显降低兔急性肠系膜缺血再灌注损伤后下腔静脉血和肠道组织中MDA、MPO水平及肠黏膜损伤。Objective To study the effect of ischemia postconditioning intervention in a rabbit＇s acute mesenteric ischemia-reperfusion injury model. Methods 120 rabbits were divided randomly into Con（ only expose SMA by operation）, I/R（ clamping SMA 30 min, reperfusing 120 min） ,IpostC1 （ clamping SMA 30 min, 3 clamping 30 s/releasing 30 s round, reperfusing 117 min）, and IpostC2 （clamping SMA 30 min,3 clamping 60 s/releasing 60 s round,reperfusing 114 min） group （n = 30）. Levels of MDA and MPO in serum and intestinal tissues were measured. Chiu-6 standard scoring was used to determine the pathology score of injured intestinal mucosae. Results Compared with the Con group, MDA and MPO levels in serum and intestinal tissues increased obviously in the three other groups, the same as in the pathology score of injured intestinal mucosae （ P 〈 0.01 ） ; Compared with the I/R group, the MDA and MPO levels in serum and intestinal tissues decreased obviously in the IpostC1 group（ P 〈 0.01 ） , but not in the IpostC2 group （ P 〉 0. 05 ）. Conclusions MDA and MPO levels in serum and intestinal tissues and intestinal mucosal injury decreased obviously in the rabbit＇s acute mesenteric ischemia-reperfusion injury model by ischemia postconditioning intervention.

关 键 词：肠系膜血管闭塞 缺血 再灌注损伤 疾病模型 动物 动物实验 

分 类 号：R657.2[医药卫生—外科学]