导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p53表达的影响  

Research of the inhibition of Daotan decoction to the expression of ICAM-1 and p53 in carotid atherosclerosis rabbits

作　　者：陈文强[1] 黄小波[1] 李宗信[1] 王芬[2] 王宁群[1] 

机构地区：[1]首都医科大学宣武医院,北京100053 [2]北京中医药大学附属东方医院

出　　处：《中国老年学杂志》2012年第18期3933-3935,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.30772843;No.30801481);北京市科技新星项目(2009A90);北京市中医药科技项目(JJ2009-037)

摘　　要：目的研究导痰汤是否能通过对内皮细胞黏附分子-1(ICAM-1)和p53通路的调节而干预颈动脉粥样硬化。方法通过免疫组化方法,观察导痰汤对颈动脉硬化家兔血管ICAM-1和p53表达的影响。结果模型组血管内皮细胞、平滑肌细胞的胞浆中有ICAM-1和p53强阳性表达,显著高于对照组(P<0.01)。导痰汤治疗组细胞内ICAM-1和p53阳性表达显著低于模型组(P<0.01),且随剂量的增加,导痰汤对ICAM-1和p53的抑制逐渐增强;而p53与ICAM-1水平呈显著正相关(r=0.809,P<0.01)。结论导痰汤可以有效抑制颈动脉硬化家兔血管内皮细胞中ICAM-1和p53的表达。Objective To research the influence of Daotan decoction on the expression of ICAM-1 and p38 in carotid atherosclerosis rabbits. Methods Expressions of ICAM-1 and p53 were observed by immunol-histochemistry. Results Compared with control group, the expressions of ICAM-1 and p53 in model group were significantly increased （P 〈 0.01 ）. And compared with model group, Daotan decoction could significantly restrain ICAM-1 and p53 expressions （ P 〈 0. 05 ）. There was significantly positive relationship between the expressions of ICAM-1 and p53 （r = 0. 809, P 〈 0. 01 ）. Conclusions Daotan decoction could restrain ICAM-1 and p53 expressions in carotid atheroscle- rosis rabbits.

关 键 词：导痰汤 脐静脉内皮细胞 细胞间黏附分子-1 P53 

分 类 号：R285[医药卫生—中药学] R543.5[医药卫生—中医学]