自拟复方中药对PD多巴胺能神经元保护作用的研究  

Neuroprotective effect of a new formulated traditional Chinese medicine in the MPTP model mice with Parkinson's disease

作　　者：张丽[1] 许康[1] 张允建[2] 郭建敏[1] 

机构地区：[1]湖北省新华医院神经内科,武汉30015 [2]华中科技大学同济医学院附属协和医院,武汉430030

出　　处：《中国康复》2012年第4期246-248,共3页Chinese Journal of Rehabilitation

摘　　要：目的:观察一种新型自拟复方中药对1-甲-基-4-苯基-1,2,3,6-四氢吡啶(MPTP)所致小鼠帕金森病(PD)的预防作用及其作用机制。方法:C57BL/6J小鼠80只随机分为正常对照组(A组)、中药对照组(B组)、PD模型组(C组)、中药干预组(D组)各20只;给予B、D组中药灌胃,A、C组给予等量生理盐水灌胃,30d后给予C和D组连续5d腹腔注射MPTP造成PD模型,A和B组注射等量的生理盐水。干预结束后连续5d内检测行为学变化(爬竿试验、游泳试验)、纹状体DA浓度及一氧化氮系统(nNOS和iNOS)含量。结果:造模后第3～5天,D组较C组爬杆实验分值明显增高(P<0.05);第1～5天,D组较C组游泳实验分值明显增高(P<0.05,0.01)。造模后第5天,D组DA浓度明显高于C组(P<0.05);D组iNOS mRNA表达量明显低于C组(P<0.05)。结论:中药自拟方对小鼠PD有一定的防治作用,其机制之一可能是通过调节脑内iNOS的表达发挥作用。Objective：To investigate the neuroprotective effect of a new formulated traditional Chinese medicine （FTCM） in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine （MPTP） -- induced mouse model of Parkinson＇s dis- ease （PD）. Methods.. Eighty C57BL/6 mice were randomly divided into 4 groups： group A （normal control group）, group B （FTCM control group）, group C （PD group）, group D （FTCM plus PD group）. Mice in group B and group D were given FTCM by a gavage daily for one month, and those in group A and group C were given equivalent saline. Rats in group C and group D were injected with MPTP 30 mg/（kg, day） daily for 5 days, and those in group A and group B received equivalent saline only. In the consecutive 5 days, behavioral changes, DA in the corpus stri- atum, nNOS and iNOS were observed. Results： At 3rd to 5th day after the operation, the climb rod test score in group D was significantly higher than in group C （P〈0.05）, and at 1st to 5th day after the operation, the swim test score in group D was significantly higher than in group C （P〈0.05,0.01）. At 5th day after operation, DA in group D was significantly higher than in group C （P〈0.05）. iNOS mRNA in group D was significantly lower than in group C （P〈0.05）. Conclusion： FTCM can prevent PD rats from impairment by MPTP to some extent probably by regulating the expression of iNOS in the brain.

关 键 词：帕金森氏病 自拟复方中药 一氧化氮 MPTP 

分 类 号：R285.5[医药卫生—中药学]