利用巴西橡胶树寡核苷酸芯片筛选死皮相关基因  

Identifying the Genes Related to Tapping Panel Dryness by Hevea brasiliensis Oligonucleotide Microarrays

作　　者：李德军[1] 覃碧[1] 邓治[1] 刘向红[1,2] 

机构地区：[1]中国热带农业科学院橡胶研究所,农业部橡胶树生物学与遗传资源利用重点实验室,海南儋州571737 [2]海南大学农学院,海口570228

出　　处：《植物生理学报》2012年第9期901-908,共8页Plant Physiology Journal

基　　金：海南省自然科学基金(310072);国家自然科学基金(30960310);中国热带农业科学院橡胶研究所基本科研业务费专项(1630022011024和RC201204)

摘　　要：以健康和死皮巴西橡胶树品系热研7-33-97树皮为实验材料,利用定制橡胶树寡核苷酸芯片筛选橡胶树死皮相关基因。在橡胶树寡核苷酸芯片包含的566个基因中,死皮与健康树树皮差异表达倍数在2倍或2倍以上的有56个,占筛选转录本总数的9.9%。在56个死皮相关基因中,死皮树中上调表达基因有3个,下调表达基因有53个。这些死皮相关基因共涉及8个功能分类,"抗性及防御反应"所占比例最高,接下来是"蛋白质合成、加工及转运"和"代谢和能量",以上三类功能基因占66.07%。此外,死皮相关基因还涉及"细胞结构、生长及分化"、"细胞信号转导"、"转录相关"、"橡胶生物合成"和"未知功能"。为验证芯片结果的可信性,随机选取18个基因进行RT-PCR分析,结果表明被检测基因表达模式均与芯片结果完全一致。本研究鉴定并分析了死皮相关基因,为进一步揭示橡胶树死皮发生机制奠定了基础。With the barks of healthy and tapping panel dryness （TPD） rubber tree clone （RY7-33-97） as experi- mental materials, the genes associated with TPD were identified by custom-designed oligonucleotide microar- rays. Among 566 transcripts related to TPD of the oligonucleotide microarrays, 56 transcripts （about 9.9%） in- dicated 2-fold or higher expression differences between healthy and TPD rubber trees. Compared with healthy rubber tree, 3 and 53 genes were up- and down-regulated in TPD rubber tree, respectively. The TPD-related genes identified in the study were involved in 8 functional classifications. Of them, ＂stress/defense response＂ was the largest classification, followed by ＂protein synthesis, process and transport＂, and ＂metabolism and energy＂, and these three classifications mentioned above represented 66.07%. In addition, the TPD-related genes were associated with ＂cell structure, growth and development＂, ＂cell signal transduction＂, ＂transcription-related＂, ＂rubber biosynthesis＂, and ＂unknown function＂. To validate the microarray results, 18 genes randomly selected were analyzed with RT-PCR. The results showed that the expression patterns of all the selected genes were con- sistent with ones from microarray analyses. The genes related to TPD were identified by oligonucleotide mi- croarrays, which laid foundations for elucidating the molecular mechanism underlying TPD in rubber tree.

关 键 词：巴西橡胶树 树皮 死皮分子机制 死皮相关基因 寡核苷酸芯片 

分 类 号：S794.1[农业科学—林木遗传育种]