IL-17对小鼠心肌成纤维细胞Ⅰ/Ⅲ胶原表达的作用研究  

Effect of IL-17 on collagen Ⅰ/Ⅲ expression in cardiac fibroblasts isolated from BALB/c mice

作　　者：苏兆亮[1] 王映梅[2] 刘嫣方[1] 汪汀[1] 田莎莎[1] 张盼[1] 纪晓昀[1] 马珂[1] 许化溪[1] 

机构地区：[1]江苏大学免疫学研究所,江苏镇江212013 [2]第四军医大学西京医院病理科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2012年第9期897-898,902,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81001319);中国博士后基金(2012M-511705);江苏省博士后基金(1102129C);江苏大学高级人才启动基金(11JDG128)

摘　　要：目的:探讨IL-17对小鼠心肌成纤维细胞Ⅰ/Ⅲ胶原蛋白表达的影响及其分子机制。方法:从7～14 d BALB/c小鼠心脏中分离培养心肌成纤维细胞,分别用100 ng/mL IL-17刺激0、24、48、72 h,PCR检测其表面IL-17受体、免疫荧光检测I/III型胶原表达水平,Western blot法检测PKCβ、Erk1/2、NF-κB磷酸化水平。结果:心肌成纤维细胞表面有IL-17RA/C的表达;IL-17刺激心肌成纤维细胞24 h后Ⅰ/Ⅲ型胶原表达明显增加,Western blot显示IL-17刺激心肌成纤维细胞后分别在30、45、45 min导致PKCβ、Erk1/2、NF-κB的磷酸化。结论:IL-17可以诱导心肌成纤维细胞Ⅰ/Ⅲ型胶原的表达,其分子机制可能是PKCβ-ERK1/2-NF-кB依赖性的。AIM：To investigate the effect of IL-17 on the expression of collagen Ⅰ/Ⅲ in cardiac fibroblasts and analyze its molecular mechanism.METHODS： Cardiac fibroblasts were isolated from 7-14-day-old BALB/c mice and cultured in DMEM with 10% fetal bovine serum（FBS）.The cells were collected after IL-17 treatment for 0,24,48,72 h.IL-17 receptors on cardiac fibroblasts were detected by PCR;the collagen Ⅰ/Ⅲ expression was analyzed by immunofluorescence;the PKCβ,Erk1/2,NF-κB phosphorylation were investigated by Western blotting.RESULTS： IL-17RA/C was expressed on cardiac fibroblasts;after 24 h of IL-17 stimulation,the collagen Ⅰ/Ⅲ expression obviously increased;Western blotting showed that PKCβ,Erk1/2 and NF-κB were phosphorylated on 30,45,45 min,respectively.CONCLUSION： IL-17 could induce the expression of collagen Ⅰ/Ⅲ in cardiac fibroblasts,which might be related with PKCβ-ERK1/2-NF-кB phosphorylation.

关 键 词：IL-17 心肌成纤维细胞 心肌纤维化 

分 类 号：R392-33[医药卫生—免疫学] R363.21[医药卫生—基础医学]