薏苡仁提取物注射液对人肺腺癌的抗肿瘤作用研究  

Anti-tumor effect of coix seed extract injection on the human lung adenocarcinoma

作　　者：严萍萍[1] 尹蓓珮[1] 刘畅[1] 陆宏祺[1] 李炳生[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200437

出　　处：《世界临床药物》2012年第9期533-538,共6页World Clinical Drug

基　　金：国家"重大新药创制"科技重大专项资助(项目编号:2009ZX09301-007)

摘　　要：目的研究薏苡仁提取物注射液(康莱特,Kanglaite)对人肺腺癌体外及体内的抗肿瘤作用。方法运用CCK-8细胞增殖试验、划痕试验以及Transwell小室穿膜试验考察康莱特对A549细胞株的增殖、迁移及侵袭的影响:建立人肺腺癌A549裸鼠异种移植模型,考察高、中和低剂量静脉给药的康莱特单用或顺铂合用的抗肿瘤作用。结果体外试验显示,经康莱特作用后,A549细胞的增殖、迁移和侵袭均受到明显抑制。康莱特高、中和低剂量静脉给药,对人肺腺癌A549裸鼠异种移植模型的肿瘤相对增殖率分别为34.52%、36.67%和42.47%;高、中和低剂量康莱特合并低剂量顺铂的肿瘤相对增殖率分别为26.75%、22.48%及29.82%。结论康莱特在体外及体内对人肺腺癌均有良好的抗肿瘤活性。康莱特与低剂量顺铂合用,体内抗肿瘤作用增强。Objective To evaluate the anti-tumor effect in vitro and in vivo of coix seed extract injection （Kanglaite） on the human lung adenocarcinoma. Methods A549 cell line was treated with Kanglaite. CCK-8 cell viability assay, scratch assay and transwell assay were performed to investigate the effect of Kanglaite on cell proliferation, migration and invasion. Xenografi in nude mice of human lung adenocarcinoma A549 were established and then treated with Kanglaite alone or combined with chemotherapeutic agent in order to evaluate their anti-tumor effect in vivo. Results Kanglaite suppressed the cell proliferation, migration and invasion of A549 cell line. After intravenous drug administration with Kanglaite at the dose of high, medium and low concentration, the relative tumor proliferation rate in nude mice of human lung adenocarcinoma A549 was 34.52%, 36.67% and 42.47%; after treatment of Kanglaite in different doses combined with low dose of chemotherapeutic agent respectively, the relative tumor proliferation rate was 26.75%, 22.48% and 29.82%. Conclusion Kanglaite possesses well acknowledged anti-tumor activity in vitro and in vivo. The anti-tumor effect in vivo was enhanced after Kanglaite combined with low dose of chemotherapeutic agent.

关 键 词：薏苡仁提取物 非小细胞肺癌 肺腺癌 A549细胞 抗肿瘤 康莱特 

分 类 号：R285[医药卫生—中药学] R734.2[医药卫生—中医学]