新型冷冻管承载设计对人类精子复苏率的影响  

Effects of the novel cryovial vector on cryosurvival rate of human semen

作　　者：邹沙沙[1] 陈婷婷[1] 王亚楠[1] 施文博[1] 王如瑶[1] 刘勇[1] 曹小蓉[1] 李铮[1] 胡洪亮[1] 

机构地区：[1]上海交通大学医学院附属仁济医院泌尿外科,上海市男科学研究所精子发育与遗传学实验室,上海市人类精子库,上海200001

出　　处：《中国男科学杂志》2012年第7期19-22,共4页Chinese Journal of Andrology

基　　金：基金项目资助：国家重点基础研究发展计划（2010CB945200,2011CB944504）;上海市教委重点课题（10ZZ70）;上海交通大学自然基金课题（2008XJ017）;2010上海交通大学医工联合项目（YG2010MS27）

摘　　要：目的探讨精液冷冻管承载工具对人类精液活动力及复苏率的影响，优化精子冷冻保存的方案。方法取本库20例健康自愿供精的精液，同一例供精者的精液分成两份：实验组冷冻管用棉花包裹，对照组不做任何处理。通过相差显微镜镜检，利用计算机辅助精液分析（CASA）系统分析两组冷冻前后人类活动精子的运动参数，并作相关的统计学分析及比较。结果实验组冷冻复苏率为（66．71±6．51）％，对照组冷冻复苏率为（59．67±7．74）％（平均提高了7．04％）（P〈0．01）。结论冷冻管包裹棉花的冷冻前处理对精子有一定的保护作用，因而冷冻管承载工具的专门设计对精液冷冻有实质性意义。Objective To explore the effects ofcryovial vector on the cryosurvival of human spermatozoa and optimize the protocol of cryopreservation of human semen. Methods Semen samples were obtained from 20 healthy volunteer donors, and each one was aliquoted equally and transferred into the experimental cryotube wrapped with cotton （the experiment group） and the non-treated cryotube （the control group） respectively. All control and post-thaw samples were assessed by a computer-assisted semen analyzer（CASA） with a phase contrast microscope for determination of sperm motility parameters. Some statistical methods were used for sperm motility parameter comparison between the control and the experimental group. Results The recovery rate of sperm motility （66.71 ±6.51）% in the experimental group wassignificantly higher than that of the control group （59.67 ±7.74）%, （P〈0.01）. Conckution The cryotube wrapped with cotton showed some protective effects on cryosurvival of sperm. Therefore, it might be critical to design a special storage instrument for sperm cryopreservation.

关 键 词：精液保存 精子能动性 

分 类 号：R698.2[医药卫生—泌尿科学]