“突尼斯软籽”石榴叶片愈伤组织再生体系的建立  

Establishing of callus regeneration system from leaf in Tunisia soft-seed pomegranate

作　　者：陈延惠[1,2] 胡青霞[1,2] 谭彬[1,2] 连红可[1] 马海旺 李洪涛 冯建灿[1,2] 

机构地区：[1]河南农业大学,河南郑州450002 [2]河南省果树瓜类生物学重点实验室,河南郑州450009 [3]郑州郑研种苗科技有限公司,河南郑州450015 [4]郑州市城市园林科学研究所,河南郑州450003

出　　处：《经济林研究》2012年第2期83-87,共5页Non-wood Forest Research

基　　金：河南省科技攻关项目(072102150001);郑州市科技攻关项目(083SGYG24123-6)

摘　　要：为了石榴遗传转化研究的开展,以"突尼斯软籽"石榴为研究材料,叶片作为外植体,研究了不同灭菌时间和不同生长调节物质对离体叶片愈伤组织再生的影响。结果表明:最适宜的暗培养时间为14 d,最佳愈伤组织分化培养基为MS+BA 1.0 mg.L-1+NAA(0.3～0.5)mg.L-1,最佳不定芽分化培养基为MS+NAA 0.3 mg L-1+TDZ 1.5 mg L-1+KT(1.5～2.0)mg L-1,最佳新梢增高的培养基为MS+NAA 1.0 mg L-1+BA 0.2 mg L-1+KT 1.0 mg L-1,适宜生根的培养基为MS+NAA0.5 mg L-1,生根率达100%。In order to develop genetic transformation test of pomegranate, using leaves in Tunisia soft-seed pomegranate as explants, the effects of different sterilization time and different growth regulators on leaf regeneration in vitro were studied. The results showed that the optimum dark culture time was 14 days. The optimum medium for callus differentiation was MS supplemented with BA 1.0 mg-L1 and NAA （0.3-0.5） mg＇L1. The optimum medium for adventitious buds differentiation was MS supplemented with NAA 0.3 mg·L-1, TDZ 1.5 mgoL1 and KT （1.5-2.0） mgoL1. The optimum medium for shoot growth was MS supplemented with NAA 1.0 mg·L-1, BA 0.2 mg·L-1 and KT 1.0 mg·L-1. The best medium for rooting was MS supplemented with NAA 0.5 mg·L-1, and rooting rate reached 100%.

关 键 词：“突尼斯软籽”石榴 愈伤组织 再生体系 

分 类 号：S665.4[农业科学—果树学]