维生素D类似物EB1089对肝癌细胞增殖凋亡侵袭的影响  

Effects of vitamin D analogues EB1089 on proliferation, apoptosis and invasion of heptocellular cells

作　　者：盛茂林[1,2] 许戈良[1,2] 荚卫东[1,2] 任维华 刘文斌[1,2] 张传海[1,2] 周腾[1,2] 

机构地区：[1]安徽医科大学附属省立医院肝脏外科,合肥230001 [2]安徽医科大学附属省立医院肝胆胰外科安徽省重点实验室,合肥230001

出　　处：《安徽医科大学学报》2012年第10期1174-1178,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30972893);安徽省"115"产业创新团队项目

摘　　要：目的探讨维生素D类似物EB1089对肝癌细胞株增殖、凋亡和侵袭的影响。方法体外培养肝癌细胞株MH-CC97H,用不同浓度的EB1089分别培养不同的时间段。用四唑蓝比色实验(MTT)检测细胞的存活和生长,用流式细胞仪检测细胞的凋亡,用Transwell和划痕实验检测细胞的侵袭迁移能力,用RT-PCR方法检测雌激素受体α(ERα)、核转录因子p65(NF-κB p65)和基质金属蛋白酶-9(MMP-9)的mRNA的表达。结果 EB1089可以抑制肝癌细胞株的增殖,诱导肝癌细胞发生凋亡,抑制肝癌细胞的侵袭迁移。同时,EB1089增加MHCC97H细胞中ERαmRNA表达,下调NF-κB p65和MMP-9 mRNA表达。结论 EB1089可能通过ERα途径抑制肝癌细胞株的增殖、侵袭迁移能力,诱导其凋亡。To investigate the effects of vitamin D analogue EB1089 on the proliferation, apoptosis, invasion and migration of heptocellular cells. Methods MHCC97H heptocellular cell lines were treated with EB1089 of different concentration in different period of time. The conditions of cell proliferation was analyzed by MTF methods. The cell apoptosis was analyzed by flow cytometer. The conditions of cell migration and invasion were analyzed by wound healing assays as well as transwell assays. The expression of ERa, NF-KB p65 and MMP-9 mRNA in cells were tested by RT-PCR methods. Results EB1089 could inhibit the proliferation, invasion and induce apoptosis of heptocellular cell lines. Meanwhile, EB1089 increased the expression of ERa mRNA and decreased the expression of NF-KB p65 and MMP-9 mRNA. Conclusion EB1089 may inhibit the proliferation, invasion and induce apoptosis of heptocellular cell lines via ERα

关 键 词：维生素D 肝癌 雌激素受体Α 

分 类 号：R977.24[医药卫生—药品] R735.7[医药卫生—药学]