重症急性胰腺炎大鼠结肠黏膜下神经节一氧化氮合酶阳性神经元及乙酰胆碱转移酶阳性神经元的改变  

Changes of nitric oxide synthase immunoractive neurons and choline acetyltransferase immunoractive neurons in colonic submucous ganglia of severe acute pancreatitis rats

作　　者：林中[1] 袁园[1] 李小玲[1] 程晓雯[1] 张诚[1] 郑清华[1] 

机构地区：[1]桂林医学院附属医院消化内科,桂林541004

出　　处：《安徽医科大学学报》2012年第10期1185-1189,共5页Acta Universitatis Medicinalis Anhui

基　　金：广西壮族自治区卫生厅中医药科技专项课题(编号:GZ-KZ10-125)

摘　　要：目的探讨伴有胃肠动力障碍的重症急性胰腺炎(SAP)大鼠结肠黏膜下神经节一氧化氮合酶(NOS)阳性神经元及乙酰胆碱转移酶(ChAT)阳性神经元的改变。方法20只SD大鼠随机均分为假手术组与SAP组,用逆行胰胆管注射牛磺胆酸钠的方法制作大鼠SAP模型。24 h后,测定假手术组与SAP组小肠推进比、结肠蠕动频率、粪便含水量、胰腺病理评分及结肠蠕动频率与NOS、ChAT表达比例相关性分析。制备结肠黏膜下神经节全层标本,运用免疫荧光技术观察两组黏膜下神经节,NOS阳性神经元及ChAT阳性神经元的改变。结果与假手术组相比,SAP组结肠蠕动频率减慢(P<0.01)、小肠推进比减少(P<0.01)、粪便含水量降低(P<0.01),胰腺病理评分增高(P<0.01),其结肠黏膜下神经节中ChAT阳性神经元表达下调(43.85%±13.58%vs 16.27%±6.97%,P<0.05),但NOS阳性神经元无明显改变(22.49%±5.14%vs 24.36%±4.79%,P>0.05)。相关性分析显示ChAT阳性神经元与结肠蠕动频率呈正相关(r=0.738,P<0.01)。结论 SAP伴有胃肠动力障碍,其发病机制可能与ChAT阳性神经元或其他神经元的重塑有关。Objective To investigate the changes of NOS-IR neurons and ChAT-IR neurons in colonic submucous ganglia of SAP rats with gastrointestinal dysmotility. Methods Twenty SD rats were randomly divided into two groups： sham operated group, SAP group. SAP model was induced by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. 24 h after SAP induction, small intestinal transit index, frequency of colonic peristalsis, water content of the fecal matter, score of pancreas lesion were measured. The correlations between the frequency of colonic peristalsis and percentages of ChAT-IR neurons and NOS-IR neurons were analyzed. Wholemount preperations of colonic submucous ganglia were prepared and double immunoflouorescence for HuC/HuD protein and NOS/ChAT was employed to observe NOS-IR neurons and ChAT-IR neurons in colonic submucous ganglia. Results Compared with sham operated group, small intestinal transit index, frequency of colonic peristalsis and water content of fecal matter were significantly lower （P 〈 0. 01 ）. Score of pancreas lesion was higher in SAP group （P 〈 0. 01 ）. Compared with sham operated group, percentage of ChAT-IR neurons was significantly lower in SAP group （43.85% ± 13.58% vs 16. 27% ± 6.97% ,P 〈0.01 ）. However, there was no significant change in NOS-IR neurons between two groups （22.49% ± 5.14% vs 24. 36% ± 4. 79% , P 〉 0. 05 ）. Correlative analysis showed that there was a positive correlation between the percentage of ChAT-IR neurons and the frequnency of colonic perstalsis（r = 0. 738, P 〈 0.01 ）. Conclusion The mechanism of underlying gastrointestinal dysmotility in SAP rats may be related with the plasticity of ChAT-IR neurons, or other neurons that govern the peristalsis of intestine.

关 键 词：重症急性胰腺炎 黏膜下神经节 一氧化氮合酶阳性神经元 乙酰胆碱转移酶阳性神经元 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R576[医药卫生—基础医学]