拉米夫定治疗慢性乙型肝炎发生病毒学反弹患者YMDD变异分析  

Analysis of YMDD mutation in chronic hepatitis B patients with virologic breakthrough after lamivudine treatment

作　　者：方立庆[1] 张振华[1] 梁佳佳[1] 杨玥[1] 孙丙虎[1] 张黄醒[1] 赵小苗[1] 张亚飞[1] 李旭[1] 

机构地区：[1]安徽医科大学第一附属医院感染病科,合肥230022

出　　处：《安徽医科大学学报》2012年第10期1265-1267,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省卫生厅医学科研课题(编号:09A069;2010C053);高等学校博士学科点专项科研基金(编号:20093420120005)

摘　　要：选取402例服用拉米夫定后出现乙型肝炎病毒(HBV)DNA反弹的慢性乙型肝炎(CHB)患者血清样品,应用荧光PCR法定性检测YMDD变异情况。402例中有309例(76.87%)检测出YMDD变异,其中120例为YVDD变异,74例为YIDD变异,115例为YVDD+YIDD联合变异;309例YMDD变异患者中同时检测出变异株与野生株占69.26%(214/309),仅检测出变异株占30.74%(95/309)。另外,93例为YMDD野生型阳性。402 serum samples were collected from chronic hepatitis B（CHB） patients with HBV DNA rebounding after lamivudine treatment from outpatient clinic and wards of infection department. YMDD variations were detected by using fluorescent PCR. The variants with YMDD mutation were 309 out of the 402 patients（76. 87% ） ,mutation patterns detected in these patients included 120 YVDD ,74 YIDD and 115 mixed type （YIDD ＋ YVDD）. Of all 309 YMDD mutations detected, the YMDD mutant HBV strains coexisting with wild-type virus accounted for 69.26% （214/309） and only YMDD mutant HBV strains 30. 74% （95/309）. Besides, 93 out of the 402 patients were only detected wild-type virus.

关 键 词：乙型肝炎病毒 变异 拉米夫定 YMDD 

分 类 号：R512.62[医药卫生—内科学]