采用经姜黄素处理的未成熟树突状细胞延长大鼠移植肾存活时间  

Prolongation of the survival of rats renal ailografts by using tolerogenic dendritic cells treated with curcumin

作　　者：江志清[1] 张孝斌[1] 吴天鹏[1] 

机构地区：[1]武汉大学人民医院外科,430060

出　　处：《中华器官移植杂志》2012年第9期563-566,共4页Chinese Journal of Organ Transplantation

摘　　要：目的探讨姜黄素（Cur）处理的树突状细胞（DC）诱导同种T淋巴细胞低反应性的效果以及对大鼠移植肾存活时间的影响。方法体外培养Wistar大鼠骨髓来源的DC，经Cur处理后，以流式细胞仪检测细胞CDllc、CD80、CD86及主要组织相容性复合物（MHC）1I类抗原的变化，酶联免疫吸附试验测定DC分泌白细胞介素12（IL-12）的水平，混合淋巴细胞反应（MLR）检测其刺激Lewis大鼠T淋巴细胞增殖的能力，二次MLR测定其诱导的T淋巴细胞抗原特异性低反应性。以Wistar大鼠为供者，Lewis大鼠为受者，进行肾移植。术前第7天，经尾静脉给受者输注用Cur处理的供者DC，分设不处理对照组和未成熟DC对照组（经尾静脉注射供者的未成熟DC），术后观察移植肾存活时间及组织学改变情况，第14天检测受者T淋巴细胞对供者成熟DC的反应性。结果Cur能明显抑制DC共刺激分子CDl1c、CD80、CD86及MHCⅡ类抗原的表达以及IL-12的分泌（P〈0．05）。同种T淋巴细胞对经Cur处理过的DC刺激的增殖能力明显减低，且这种低反应性具有抗原特异性。对照组和未成熟DC对照组移植肾的存活时间分别为（8．6±2．1）d和（22．4±7．4）d，实验组为（31．5±6．9）d，实验组移植肾存活时间明显长于对照组和未成熟DC对照组（P〈0．05），且其移植肾组织的损伤程度最轻。实验组受者的T淋巴细胞对供者成熟IX2刺激的反应性明显低于对照组（P〈0．05），而对第三方无关抗原的刺激保持较高增殖强度。结论Cur能抑制DC成熟功能，诱导供者特异性的T淋巴细胞低反应性，移植前输注经Cur处理的未成熟DC能显著延长大鼠移植肾的存活时间。Objective To study the efficacy of hypo-responsiveness of allogenic T-cells induced by curcumin （Cur）-treated dendritic cells （DCs） and influence on survival time of renal allografts in rats. Methods DCs were generated from Wistar rat bone marrow and treated with Cur. The costimulatory molecuIes （CD11c, CD80, CD86 and major histocompability complex 1] ） were determined by using flow cytometry, and the production of IL-12 in DCs culture supernatant was examined by using ELISA. The probability of Cur-treated DCs to stimulate the proliferation of Lewis rat T cells was detected by using mixed leukocyte reaction （MLR）, and the antigen-specific T cell hypo-responsiveness was analyzed by using secondary MLR. Allograft renal transplantation animal models were established by using Wistar rats as donors, and Lewis rats as recipients. At 7th day before allograft renal transplantation, Cur-treated DCs from donors were injected into recipients through tail vein, meanwhile the non-treated control group and immature DCs control group （immature DCs from donors were injected into recipients through tail vein） were set up. The allograft survival time and allograft pathology after transplantation were assayed. Reaction of T ceils from the recipients to mature DCs of donors was analyzed at 14th day. Results Cur restrained the expression of DCs phenotype and production of IL-12 （P〈0. 05）. Cur-treated DCs displayed poor ability to stimulate T cells proliferation, and potential to induce antigen-specific T cell hypo-responsiveness. The survival time of the renal allograft in Cur-treated CDs group [（31.5±6. 9） days] was significantly longer （P〈0. 05） than in control group [（8.6± 2. 1 ） days] and immature DCs control group [（22. 4 ± 7. 4） days], and the pathological lesions in the renal allografts in Cur-treated CDs group were milder than in the control group and immature DCs control group. T cells from the recipients injected with Cur-treated DCs showed significant hypo-responsiveness to ma

关 键 词：姜黄素 树突状细胞 移植免疫学 肾移植 

分 类 号：R392[医药卫生—免疫学]