PPARα和PPARγ基因在不同脂尾型绵羊脂肪组织中的发育性表达研究  

Developmental Expression of PPARα and PPARγ mRNA in Adipose Tissues of Different Fat-tailed Sheep

作　　者：林婄婄[1] 高中元[1] 袁亚男[1] 武建亮[1] 刘宝凤[1] 周沙沙[1] 乔利英[1] 刘建华[1] 梁琛[1] 刘文忠[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2012年第9期1369-1376,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30972084);山西省科技攻关项目(011029)

摘　　要：为研究PPARα和PPARγ基因在不同脂尾型绵羊脂肪组织中的发育性表达规律,以探讨这2个基因与绵羊脂肪代谢的关系。本研究以2个具有显著尾型差异的绵羊品种(广灵大尾羊和小尾寒羊)为研究对象,用实时荧光定量方法研究PPARα和PPARγ基因在2、4、6、8、10和12月龄个体的7种脂肪组织(大网膜、小网膜、尾部脂肪、皮下脂肪、肠系膜、肾周脂肪、腹膜后脂肪)中的mRNA的表达。结果表明,PPARα和PPARγ在各脂肪组织中都有表达。总体而言,浅层脂肪组织中的表达量低于深层脂肪组织。作为主效应,品种和月龄基本不影响2个基因的mRNA表达。尽管性别对PPARα的表达无显著影响,但在广灵大尾羊母羊中PPARγ的低表达导致性别本身及其与品种的互作达显著水平。鉴于组织间和年龄间的表达差异,这2个基因的表达具有明显的空间性,但时间性不明显。尽管二者在调节脂肪代谢方面的功能相反,但是存在协同作用。The objectives of this study were to investigate the developmental expression character- istics of PPARα and PPARγ mRNA in two sheep breeds of distinct tail types and to discuss the probable relationship between the mRNA expression and metabolism of adipose tissue. Guangling Large Tailed （GLT） sheep and Small Tailed Han sheep （STH） at 2, 4, 6, 8, 10 and 12 month old were used to detect the mRNA expression in seven adipose tissues （great omental, small omental, tail, subcutaneous, mesenteric, perirenal and retroperitoneal fat） by real-time PCR. The results showed that PPARα and PPARγ expressed in all tissues studied. Generally, the mR- NA expressed lower in superficial fats than that in deep fat deposits. As the main effects, months of age and breed did not basically influence the mRNA expression of PPARα and PPARγ. Though sex did not influence PPARα expression in both breeds, low expression of PPARγ in females of GLT resulted in the significant sex effect and interaction between sex and breed. Based on the expression differences between tissues and ages, respectively, the expression of the two genes was of significant spatiality but not of temporality. The two genes had a synergistic effect on the metabolism of adipose tissues despite their opposite functions.

关 键 词：绵羊 PPARΑ PPARΓ 脂肪组织 荧光定量PCR 

分 类 号：S826[农业科学—畜牧学] Q786[农业科学—畜牧兽医]