鹅DHRS7基因克隆、序列分析及其在鹅肥肝形成过程中的表达特点  

Molecular Cloning and Bioinformatics Analysis of Goose DHRS7 and Its Expression Characteristics during Goose Fatty Liver Formation

作　　者：何桦[1] 潘志雄[1] 刘贺贺[1] 李乐[1] 夏露[1] 胡深强[1] 鲁凯[1] 董霞[1] 李亮[1] 王继文[1] 

机构地区：[1]四川农业大学动物遗传育种研究所,成都611130

出　　处：《畜牧兽医学报》2012年第9期1385-1391,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家水禽产业技术体系(CARS-43-6)

摘　　要：为获得鹅DHRS7基因全长cDNA,并探讨该基因是否参与鹅肥肝的形成过程。本研究应用抑制消减杂交文库获得鹅DHRS7基因部分EST序列,采用RACE技术扩增了鹅该基因全长cDNA,并对其序列进行了生物信息分析;用荧光定量PCR技术检测了DHRS7在鹅肝脏、皮脂、腹脂等10个组织中的差异表达,以及填饲对鹅肝脏中DHRS7表达变化的影响。结果发现,鹅DHRS7基因cDNA全长1 279bp,含一个完整的开放阅读框1 011bp(可编码336个氨基酸),25bp的5′UTR和243bp的3′UTR序列;生物信息学分析结果显示,鹅DHRS7含有跨膜结构域以及NADP结合位点,且在这些功能区域变异较少;荧光定量结果显示,DHRS7在鹅肝脏和脂肪组织中都有较高表达,填饲后鹅肝脏中DHRS7的表达水平显著上调(P<0.05)。以上结果表明,DHRS7在鹅肥肝形成过程中具有重要的作用。In order to elucidate the roles of DHRS7 gene in formation of goose fatty liver, the goose DHRS7 gene full-length cDNA sequence was cloned by RACE method, and then was ana- lyzed by bioinformatics method. Meanwhile, expression of DHRS7 in goose different tissues and organs was analyzed using qRT-PCR method, as well as the mRNA expression influenced by forcing-feed was analyzed in goose liver. Results showed that the full sequence of goose DHRS7 gene was 1 297 bp, including an coding domain sequence （CDS） encoding for 336 AA, and a 25 bp 5r UTR and a 243 bp 3r UTR. Results of bioinformatics analysis showed that the geese DHRS7 posses a transmembrane region and a NADP binding region, on which functional region there is few variants. The DHRS7 had a higher expression level in geese liver and adipose tis- sues. And the expression of DHRS7 up-regulated significantly in geese liver tissues after a force feeding （P〈0.05）. The results indicate that the goose DHRS7 gene may play an important role during formation of geese fatty liver.

关 键 词：鹅 短链脱氢酶7(DHRS7) RACE CDNA全序列 生物信息学分析 

分 类 号：S835[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]