内蒙古绒山羊皮肤毛囊差异表达序列标签的筛选及分析  

Screening and Analysis of Differential Expressed Sequence Tags in Skin Hair Follicles for Cashmere Goats

作　　者：苏立宁[1,2,3] 李华[1,2] 刘东军[1] 旭日干[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010021 [2]佛山科学技术学院,佛山528231 [3]河北北方学院,张家口075000

出　　处：《畜牧兽医学报》2012年第9期1499-1503,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家转基因项目(2008zx08008-002);博士后基金资助

摘　　要：旨在筛选和分析内蒙古绒山羊与毛和绒生长相关的差异基因,本研究采用mRNA差异显示技术(DDRT-PCR)研究内蒙古绒山羊胎儿期不同日龄皮肤中控制初级毛囊和次级毛囊不同分化的差异表达序列标签。结果,共筛选了15条差异表达序列标签(dbEST登录号JK711022-JK711036),其中3条ESTs与毛囊发育有关,5条ES-Ts与次级毛囊和皮脂腺的发育有关。生物信息分析显示,JK711036与甲状腺激素受体相互作用蛋白12(TRIP12)基因有关,其他ESTs功能未知。结果表明,胎儿期初级毛囊和次级毛囊在多种代谢途径上都存在差异表达的基因,这将为进一步研究山羊毛和绒的形成分子机制奠定基础。To screen and analyze the genes associated with growth of goats＇ wool and cashmere, differential display reverse transcription polymerase chain reaction （DDRT-PCR） technology was used to study the differentially expressed sequence tags （ ESTs ） at different embryo ages in skin primary and secondary follicles of Inner Mongolia Cashmere goats. 15 ESTs were identified （Acc _N： JK711022-JK711036） , of which 3 ESTs might play a role in the development of hair follicle, and 5 ESTs might be related to secondary follicles and sebaceous glands. The results of bioinfor- matics analysis showed that JK711036 shared homology with other species＇ TRIP12 gene, but the function of other ESTs were unknown. All these were showed that many differential expressed genes was associated with primary and secondary wool follicles on metabolism pathways, which would establish molecular foundation for the formation of wool and cashmere in goats.

关 键 词：绒山羊 毛囊 DDRT-PCR 表达序列标签 

分 类 号：S827[农业科学—畜牧学] Q523.1[农业科学—畜牧兽医]