孕酮对全脑缺血/再灌注损伤大鼠学习记忆及海马P2X_7受体表达的影响  

Effects of progesterone on learning and memory and P2X_7 receptor expression in the hippocampus after global cerebral ischemia/reperfusion injury in rats

作　　者：李新娟[1] 何瑞芳[1] 李爽[1] 李晓娟[1] 李东亮[1] 

机构地区：[1]新乡医学院生理学与神经生物学教研室,河南新乡453003

出　　处：《中国应用生理学杂志》2012年第5期472-475,共4页Chinese Journal of Applied Physiology

基　　金：河南省科技攻关项目资助课题(No 092102310098;No 092101310600)

摘　　要：目的:研究孕酮(PROG)对全脑缺血/再灌注(I/R)损伤大鼠学习记忆及海马P2X7受体表达的影响。方法:雄性SD大鼠48只,随机分成4组(n=12):正常组、假手术组、I/R组和I/R+PROG组。采用改良Pulsinelli’s 4血管闭塞法建立全脑缺血/再灌注损伤动物模型,Y-型迷宫检测学习与记忆成绩;免疫荧光法观察海马区P2X7受体蛋白表达;羟胺氧化法测定海马组织中超氧化物歧化酶(SOD)活性;硫代巴比妥酸法测定海马组织中丙二醛(MDA)含量。结果:正常组和假手术组学习记忆成绩、海马区P2X7阳性表达细胞数、海马组织中SOD活性和MDA含量差异无显著性;与假手术组相比,I/R组学习记忆成绩显著降低(P<0.01);与I/R组相比,I/R+PROG组学习记忆成绩显著升高(P<0.05)。与假手术组相比,I/R组海马区P2X7阳性表达细胞数显著增多(P<0.01);与I/R组相比,I/R+PROG组海马区P2X7阳性表达细胞数显著减少(P<0.01)。与假手术组相比,I/R组海马组织中SOD活性显著降低(P<0.01),而MDA含量显著升高(P<0.01);与I/R组相比,I/R+PROG组海马组织中SOD活性显著升高(P<0.05),而MDA含量显著降低(P<0.01)。结论:PROG可明显改善全脑缺血/再灌注损伤大鼠学习与记忆能力,其作用机制可能与下调海马区P2X7受体蛋白表达和清除氧自由基有关。Objective： To explore the effects of progesterone（PROG） on learning and memory and P2X7 receptor expression in the hippocampus after global cerebral ischemia/reperfusion（I/R） injury in rats.Methods： Forty-eight male SD rats were randomly divided into four groups（n=12） that were normal group,sham-operated group,I/R group and I/R＋PROG group.The global cerebral I/R injury models were established by improved Pulsinelli’s four vessel occlusion,the learning and memory were evaluated by Y-maze,the expression of P2X7 receptor protein in the hippocampus were detected by the immunofluorescence,the activity of superoxide dismutase（SOD） in the hippocampus were detected with hydroxylamine oxidation method,the content of malondialdehyde（MDA） in the hippocampus were detected with penthiobarbital acid method.Results： There was no significant difference of the learning and memory and positive expression cells of P2X7 receptor protein and the activity of SOD and the content of MDA in the hippocampus between normal group and sham-operated group.compared with sham-operated group,the learning and memory of I/R group were significantly decreased（P〈0.01）,Compared with I/R group,the learning and memory of I/R＋PROG group were significantly increased（P〈0.05）.Compared with sham-operated group,the positive expression cells of P2X7 receptor protein in the hippocampus of I/R group was significantly increased（P〈0.01）,Compared with I/R group,the positive expression cells of P2X7 receptor protein in the hippocampus of I/R＋PROG group was significantly decreased（P〈0.01）.Compared with sham-operated group,the activity of SOD in the hippocampus of of I/R group was significantly decreased（P〈0.01）,and the content of MDA in the hippocampus of I/R group was significantly increased（P〈0.01）.Compared with I/R group,the activity of SOD in the hippocampus of I/R＋PROG group was significantly increased（P〈0.05）,and the content of MDA in the hippocampus of I/R＋PROG group was significa

关 键 词：孕酮 脑缺血/再灌注 学习记忆 P2X7受体 超氧化物歧化酶 丙二醛 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R743[医药卫生—基础医学]