原发性肝癌RUNX3基因启动子区甲基化及其意义  

Methylation of RUNX3 gene promoter in HCC and its significance

作　　者：李建国[1] 江小杰[2] 

机构地区：[1]福建医科大学附属漳州市医院肝胆外科,福建漳州363000 [2]莆田学院附属医院肿瘤外科,福建莆田351100

出　　处：《第三军医大学学报》2012年第19期1933-1935,共3页Journal of Third Military Medical University

基　　金：福建省自然科学基金(2009J01326)~~

摘　　要：目的检测原发性肝癌(hepatocellular carcinomas,HCC)中人RUNT相关转录因子3(human runt-related tran-scription factor 3,RUNX3)基因启动子的甲基化情况,探讨其与患者临床特征的关系。方法收集95例HCC患者的肿瘤标本及相应的癌旁肝组织、20例正常肝组织,95例HCC患者中合并肝硬化患者57例,按照肝硬化Child-Pugh肝功能分级标准,将57例患者分为A级36例,B级11例,C级10例,运用甲基化特异性PCR检测RUNX3基因启动子CpG岛甲基化状态,分析RUNX3甲基化情况与患者临床特征的关系。结果 95例HCC组织中,45.3%(43/95)存在RUNX3基因CpG岛的异常甲基化,癌旁肝组织中有9.5%(9/95)存在异常甲基化,而正常肝组织中未检测到RUNX3基因CpG岛的异常甲基化;RUNX3基因异常甲基化在HCC组织中的发生率与癌旁组织及正常肝组织比较差异有统计学意义(P<0.01),RUNX3基因CpG岛甲基化与患者肝硬化、门脉癌栓关系密切(P<0.05)。肝硬化Child-Pugh肝功能C级组RUNX3启动子甲基化水平明显高于A级组和B级组(P<0.05)。结论 HCC存在RUNX3基因CpG岛异常甲基化,CpG岛的甲基化可能是导致其基因表达降低的主要原因之一,并与患者肝硬化、门脉癌栓密切相关。Objective To study the relation between methylation of human runt-related transcription factor 3 （RUNX3） gene promoter in hepatocellular carcinoma （HCC） and clinical characteristics of HCC patients. Methods Tumor tissue and its adjacent tissue samples were taken from 95 patients of HCC and 20 normal controls, respectively. Of the 95 HCC patients, 57 were complicated with cirrhosis and divided into group A （n =36） , group B （n = 11 ） and group C （n = 10） according to their liver function Child-Pugh score. Methylation of RUNX3 gene promoter （ CpG island） was detected by methylation-specifie PCR and its relation with the clinical characteristics of HCC patients was analyzed. Results The abnormal methylation of RUNX3 gene promoter （ CpG island） was detected in tumor tissue and its adjacent tissue samples from 45.3% （43/95） and 9.5% （ 9/95 ） patients, respectively （ P 〈 0.01 ）, but not detected in normal liver tissue samples. The methylation of RUNX3 gene promoter （CpG island） was closely correlated with cirrhosis and cancer embolus formation in portal vein （ P 〈 0.05 ）. The methylation level of RUNX3 gene promoter （ CpG island） was signifi- cantly higher in group C than in groups A and B （P 〈 0.05）. Conclusion One of the major reasons for the abnormal methylation of RUNX3 gene promoter（ CpG island） in HCC patients is the down-regulated expression of CpG island due to its methylation. Abnormal methylation of RUNX3 gene promoter（ CpG island） is closely correlated with cirrhosis and cancer embolus formation in portal vein.

关 键 词：原发性 肝细胞癌 人RUNT相关转录因子3 甲基化 

分 类 号：R894.3[医药卫生—法医学] R730.23[医药卫生—临床医学]