地表臭氧增加对黄花夹竹桃和芒果叶片膜脂过氧化程度和保护酶活性的影响  

Effects of ozone on membrane lipid peroxidation and protective enzyme activities of Thevetia peruviana and Mangifera leaves

作　　者：路光超 黄玉源[2] 陈红跃[3] 许炼烽[4] 

机构地区：[1]广州市金牛环保科技有限公司,广东广州510663 [2]仲恺农业工程学院生命科学学院,广东广州510225 [3]华南农业大学林学院,广东广州510642 [4]环境保护部华南环境科学研究所,广东广州510655

出　　处：《生态环境学报》2012年第7期1235-1240,共6页Ecology and Environmental Sciences

摘　　要：以黄花夹竹桃（Thevetia peruviana（Pers.）k.Schum.）和芒果（Mangifera indica L.）苗木为材料,研究4种臭氧（O3）体积分数[环境大气NF,φ（O3）=10×10-9~20×10-9;低体积分数处理E50,φ（O3）=50×10-9;中体积分数处理E100,φ（O3）=100×10-9;高体积分数处理E200,φ（O3）=200×10-9]下两种植物叶片膜脂过氧化程度、可溶性蛋白质质量分数、可溶性糖质量分数和保护酶活性的变化情况,旨在揭示地表臭氧体积分数升高条件下2种植物生理代谢活动变化机理及响应规律。结果表明：随臭氧体积分数的增加,2种植物丙二醛含量和膜透性均逐渐上升,且与臭氧体积分数呈显著正相关关系,说明两种植物膜脂过氧化程度加剧;低体积分数O3处理时两种植物可溶性蛋白质量分数均逐渐下降,高φ（O3）处理后小幅回升;黄花夹竹桃可溶性糖质量分数逐渐下降,芒果先上升后下降;2种植物的POD活性均显著上升,但上升幅度有较大差异;2种植物的CAT活性均呈先升高再降低的趋势。研究表明,不同体积分数的臭氧暴露下,黄花夹竹桃和芒果均受到不同程度的伤害,芒果对臭氧表现出较强的抗性,黄花夹竹桃对臭氧较敏感。Seedlings of Thevetia peruviana and Mangifera indica were applied as test materials.The seedlings were exposed to four ozone concentrations： NF（non-filtered air,φ（O3）=10×10^-9～20×10^-9）,E50（φ（O3）=50×10^-9）,E100（φ（O3）=100×10^-9） and E200（φ（O3）=200×10^-9）.Contents of malonaldehyde（MDA）,membrane permeability,contents of soluble protein and soluble sugar,activities of catalase（CAT） and peroxidase（POD） of them were determined.Reveal the physiological metabolism activities change mechanism and response pattern of the four plants under the ground surface ozone concentration rose up.The results were as followed： With concentration increasing,membrane permeability and the level of malonaldehyde（MDA） content in T.peruviana and M.indica leaves were all gradually increased and were significantly positive correlated with the concentration of O3,which indicated that the level of membrane lipid peroxidation were aggravated in the two plants.The contents of soluble protein in the two plants were all gradually declined when they exposed to low concentration of O3,while low amplitude rised when exposed to high concentration of O3（φ（O3）=200×10^-9）.The contents of soluble sugar were gradually declined in T.peruviana leaves,while the content of soluble sugar increased first and then declined in M.indica leaves.Activities of peroxidase（POD） in the two plants were all significantly increased,but the increase amplitude were different.Activities of catalase（CAT） in the two plants tended to increase first and then decline.The research shows： under different ozone concentration exposed,the two plants were both received varying degrees hurt.M.indica showed relatively strong resistance,while T.peruviana showed obviously sensitive to ozone.

关 键 词：臭氧 园林植物 膜脂过氧化 保护酶活性 污染 

分 类 号：Q945.79[生物学—植物学]