乙型肝炎病毒核酸荧光定量及血清标志物与肝功能检测的临床意义  

The clinical significance of HBV-DNA combine with liver function and HBV serm makers

作　　者：彭红波 刘亚丽 陈旭姿 

机构地区：[1]广州市番禺区何贤纪念医院检验科 [2]广州市民政局精神病院老人区 [3]广西壮族自治区桂林市75660部队门诊部

出　　处：《检验医学与临床》2012年第18期2260-2261,2263,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨乙型肝炎病毒(HBV)感染者HBV DNA检出情况及其与血清病毒标志物及肝功能在临床应用的意义。方法采用实时荧光定量聚合酶链反应(FQ-PCR)检测296例不同血清学类型的HBV感染者血清中的HBV DNA,同时检测HBV血清标志物与肝功能。结果 99例HBeAg(+)标本中HBV DNA阳性率为100%,平均HBV DNA含量为1.21×107 copy/mL,其HBV DNA检出率和含量与HBeAg(-)模式的检出情况比较差异有统计学意义(P<0.01);HBsAg(+)与HBsAg(-)模式比较,HBV DNA检测结果差异有统计学意义(P<0.01);肝功能正常组与肝功能异常组HBV DNA检出率比较差异有统计学意义(P<0.01)。58例HBsAg(-)且肝功能又正常的标本中检出了4例HBV DNA阳性。结论实时FQ-PCR定量检测HBV DNA与HBV血清标记物及肝功能指标联合应用可使诊断更准确,治疗更合理。Objective To investigate the positive rat of HBV DNA, and the value of detection with HBV DNA, sera viral marker,five index of hepatitis B and hepatic function in clinical. Methods 296 cases with HBV were detected about HBV-DNA, sera viral markers and hepatic function by real-time fluorescence quantitative PCR （FQ PCR）. Results 99 cases were diagnosed as HBeAg（T）carrying HBV-DNA（1.21 X 107 copy/mL）,and the positive rat of HBV-DNA was 100~. 4 of 58 cases with HBsAg（- ） were found to be positive HBV-DNA infection. The pos itive rat of HBV-DNA in the patients with HBeAg（- ） was significantly lower than t patients with HBeAg（ ＋ ）（P~ 0.01）. The positive rat of HBV-DNA in the patients with normal hepatic function was significantly lower than pa- tients with abnormal hepatic function（P%0.01）. Conclusion FQ-PCR is a valuable tool for the detection of HBV in the clinical,and it can be used to detect the status of HBV-DNA in a short time. It is better to diagnose type B hepatitis by HBV-DNA combined other detections,such as sera viral marker,five index of hepatitis B and hepatic function at the same time.

关 键 词：乙型肝炎病毒 HBV DNA 血清标志物 实时荧光定量聚合酶链反应 肝功能 

分 类 号：R373.21[医药卫生—病原生物学]