芙蓉菊组培快繁技术的研究  

Study on techniques of fast multiplication for the tissue culture of Crossostephium chinense

作　　者：陈雪鹃[1] 吴珏 李雪珂[1] 孙明[1,3] 张启翔[1,3] 

机构地区：[1]北京林业大学园林学院,北京100083 [2]无锡鸿源景观建设有限公司,江苏无锡214000 [3]国家花卉工程中心,北京100083

出　　处：《中南林业科技大学学报》2012年第7期100-104,127,共6页Journal of Central South University of Forestry & Technology

基　　金：中央高校基本科研业务费专项(YX2011-32);中央高校基本科研业务费专项(TD2011-27);十二五国家科技支撑计划课题(2012BAD01B07)

摘　　要：以芙蓉菊Crossostephium chinense带腋芽的茎段和叶柄为外植体进行组织培养。结果表明,带腋芽的茎段是较好的组培外植体。最佳灭菌时间为:茎段75%酒精20 s,0.1%HgCl22 min;叶柄75%酒精20 s,0.1%HgCl21 min。最适宜的初代培养基为:茎段MS+0.1 mg/L NAA+2.0 mg/L 6-BA,愈伤组织诱导率可达80.6%,叶柄MS+1.0 mg/L NAA+2.0 mg/L 6-BA,愈伤组织诱导率可达83.3%。继代培养以茎段为外植体,MS+0.2 mg/LNAA+2.0 mg/L 6-BA培养基的增值系数最高。继代苗在MS培养基中生根率为80.6%,1/2MS培养基中生根率为86.1%。By taking the stems with axillary buds and petioles as explants, the tissue culture of Crossostephium chinense was studied. The effects of different sterilization time, mediums for initial culture, multiplication culture and rooting culture were tested. The results show that the stems were the optimal explants, the best disinfection method was： the stems were treated with 75% alcohol for 20 seconds and 0.1% HgC12 for 2 minutes, the petioles were treated with 75% alcohol for 20 seconds and 0.1% HgCI2 for 1 minute; the optimal initial-generation medium was： the stems were treated with MS＋0.1 mg/L NAA＋2.0 mg/L 6-BA,the callus induction frequency was 80.6%, the stems were treated with MS＋1.0 mg/L NAA＋2.0 mg/L 6-BA, the callus induction frequency reached 83.3%; the subculture was conducted by taking the stems as the explants and with MS＋0.2 mg/L NAA＋2.0 mg/L 6-BA, thus obtaining the maximum increment coefficient. The rooting rate of subculture plantlet was 80.6% in MS medium and 86.1% in 1/2 MS medium.

关 键 词：芙蓉菊 组织培养 快繁技术 茎段 

分 类 号：S722.37[农业科学—林木遗传育种] S682.11[农业科学—林学]