米良一号猕猴桃遗传转化体系的建立  

Establishment of transformation system of Kiwifruit Miliang-1

作　　者：田宏现[1,2] 苑平[2] 王曼玲[3] 刘艺[2] 李菁[2] 夏新界[3] 谭晓风[1] 

机构地区：[1]中南林业科技大学,湖南长沙410004 [2]吉首大学,湖南吉首416000 [3]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《中南林业科技大学学报》2012年第8期81-85,共5页Journal of Central South University of Forestry & Technology

基　　金：湖南省省级科技计划(专项计划)项目"耐贮保鲜高抗性转基因猕猴桃的研究和开发"(2008JT3008);湖南省高校产学研合作示范基地开放项目(2011jsjk003)

摘　　要：为建立猕猴桃基因功能研究技术平台并通过生物技术改良猕猴桃,选用米良一号叶片和茎为外植体,通过优化培养基,建立了适合转化的高效再生系统,通过农杆菌介导将ACC(1-氨基环丙烷-1-羧酸)氧化酶基因成功转化米良一号,构建了根癌农杆菌介导米良一号遗传转化体系。结果表明,TD培养基为合适的愈伤诱导培养基,诱导率达100%,愈伤经3次继代培养转入分化培养基,分化率为90%。共获得29株潮霉素抗性植株,随机挑选14株经PCR检测,其中10株检测到ACC氧化酶基因目的条带,阳性植株占71.4%。In order to establish the technology platform for function analysis of genes and genetic improvement ofkiwifruit through biotechnology, the leaves and stems of Miliang-1 plants were used as explants. An efficient regeneration system was established for gene transformation by optimizing the culture medium. By using Agrobacterium-mediated method, transgenic plants containing the ACO （1-Aminocyclopropane-l-carboxylate oxidase） gene fragment were successfully obtained. The results show that the TD medium was the efficent medium for callus induction with a rate of 100%, After three times of subcultures, calli were moved into the differentiation medium, and the rate of differentiation was 90%. A total of 29 hygromycin resistant plants were obtained, and 14 of them were chosen randomly for PCR analysis. Ten of the analyzed plants were shown to be positive for the ACO gene insert （71.4 % ）.

关 键 词：猕猴桃 根癌农杆菌 转化体系 组织培养 

分 类 号：S663.4[农业科学—果树学]