拮抗细菌C-02防治棉花黄萎病的机理研究  

Study on Antagonistic Mechanism of Bacterium Strain C-02 against Verticillium Wilt of Cotton

作　　者：田绍仁[1] 聂太礼[1] 王梦亮[2] 杨军[1] 

机构地区：[1]江西省棉花研究所,江西九江332000 [2]山西大学应用化学研究所,太原030006

出　　处：《棉花学报》2012年第5期420-426,共7页Cotton Science

基　　金：科技部创新方法专项(2010ZM20700)

摘　　要：从抗生、竞争和诱导系统抗性三个方面对拮抗细菌C-02防治棉花黄萎病的机理展开了研究,结果表明,C-02分泌的抗菌蛋白能溶解病原菌VD-11的细胞壁,抑制其致萎毒素的分泌。与病原菌VD-11相比,C-02利用碳源有明显优势,在棉花根际土壤中具有显著的营养竞争优势和很好的定殖能力。C-02处理棉苗后,棉叶中植株防御性酶系PAL、POD和PPO的活性提高幅度大,维持时间长。C-02防治棉花黄萎病的机理是抗生、溶菌、竞争和诱导棉苗系统抗性的综合表现。The mechanisms of antagonists against fungal pathogens have become increasingly important in biological control in recent decades. In this paper, the mechanisms of antagonism, competition, and systemic-resistance induction in the bacterium strain C-02 against Verticillium wilt of cotton were studied by double culture, liquid fermentation, and inducing cotton seedlings, respectively. The results indicated that an antifungal protein from strain C-02 could destroy the cell wall of pathogen strain VD-11 and inhibit its toxin secretion. The results also showed that strain C-02 could colonize the rhizospheric soil of cotton fields better than VD-11, and they had the advantage in carbon resource use. When cotton leaves were treated with a bacterial culture of C-02, PAL, POD and PPO activity was quickly enhanced and remained at high levels for a long time. In conclusion, the antagonistic mechanisms of C-02 against Verticillium wilt of cotton form a comprehensive behavior including antagonism, bacteriolysis, competition, and systemic-resistance induction.

关 键 词：拮抗细菌 棉花 黄萎病 机理 致萎毒素 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]