龙眼叶片活性氧代谢和抗氧化系统对La^(3+)的响应  

Responses of Reactive Oxygen Metabolism and Antioxygen System of Longan Leaf to La^(3+)

作　　者：李永裕[1] 潘腾飞[1] 余东[2] 邱栋梁[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建省农科院果树研究所,福建福州350013

出　　处：《中国稀土学报》2012年第5期612-619,共8页Journal of the Chinese Society of Rare Earths

基　　金：国家自然科学基金(30400061)资助;福建省自然科学基金(2011J01082)资助

摘　　要：以一年生龙眼小苗为材料,研究了La3+对龙眼叶片活性氧代谢和抗氧化系统的影响,探讨La3+提高植物抗逆境能力作用机制。龙眼小苗喷施10,20,30,40,50 mg.L-1的La(NO3)3两次,通过检测叶片的自由基含量、抗氧化酶活性和抗氧化物质含量来分析La3+提高龙眼抗氧化的作用。结果表明:20～30 mg.L-1La3+处理可明显清除龙眼叶片的.O2-和H2O2,减少MDA生成量;显著提高叶片SOD和CAT酶活性,降低POD活性,提高AsA-GSH循环运行效率及相关代谢酶APX,MDAR,DHAR和GR的活性,保证AsA和GSH再生,显示稀土离子La3+对龙眼抗性具有显著的正调节作用。The effects of La3 ＋ on active oxygen metab- olism and antioxygen system of leaf of one year old longan were studied to investigate the mechanism of enhancing tolerance to negative environment of plant treated by La3＋ The longan leaves were treated by 10, 20, 30, 40, 50 mg-L-1 La（NO3）3 for two times, and the contents of free radicals and antioxidant, activities of antioxidase were determined to analyze the role of La3＋ on stress tolerance of longan. The results showed that the treatments of La3＋ ranged from the concentrations of 20 to 30 mg·L-1 scavenged ·O2- and H2O2 markedly, reduced MDA content, signifi- cantly increased activity of SOD and CAT, decreased POD activity, and ensured the regeneration of AsA and GSH by increasing operation efficiency of AsA- GSH cycle and activities of corresponding enzymes of APX, MDAR, DHAR and GR, which suggested the significant influence of rare earth element La3＋ on the resistance of longan.

关 键 词：La(NO3)3 龙眼 活性氧 抗氧化系统 稀土 

分 类 号：S667.201[农业科学—果树学] Q945.78[农业科学—园艺学]