反油酸对人脂肪细胞因子表达的影响  

Effect of elaidic acid on gene expression of human adipokines

作　　者：白英龙[1] 郭洁[1] 任丽娜[2] 孟涛[2] 刘福定[1] 翟玲玲[1] 

机构地区：[1]中国医科大学公共卫生学院,辽宁沈阳110001 [2]中国医科大学附属第一医院产科

出　　处：《环境与健康杂志》2012年第9期771-773,F0003,共4页Journal of Environment and Health

基　　金：国家自然科学基金(81172691)

摘　　要：目的观察反油酸(elaidic acid,EA)对人成熟脂肪细胞因子表达的变化,探讨其对人脂肪细胞内分泌功能的可能影响。方法将人脂肪组织来源间质干细胞(human adipose tissue-derived stromal cells,hADSCs)经体外原代培养,诱导分化为成熟脂肪细胞,分别于0(对照)、1、5和25μmol/L的EA暴露72 h。采用实时定量PCR(real time PCR)方法检测成熟脂肪细胞因子瘦素(leptin)、脂联素(adiponectin)和抵抗素(resistin)mRNA的表达水平。结果与对照组比较,各剂量EA暴露组人成熟脂肪细胞脂联素mRNA的表达水平均较高,仅25μmol/L EA暴露组人成熟脂肪细胞抵抗素mRNA的表达水平均较低,差异均有统计学意义(P<0.05);而各剂量EA暴露组人成熟脂肪细胞瘦素mRNA的表达水平均无显著变化。结论人脂肪细胞因子对外源性干预物EA的反应不一致,EA具有上调脂联素mRNA表达、下调抵抗素mRNA表达的作用。Objective To compare the difference of adipokines gene expression induced by elaidic acid （EA）, and investigate the potential effect of trans fatty acids （TFAs） on endoucrinal function of human adipocytes. Methods Human adipose tissue-derived stromal cells （hATSCs） were cultured primarily,then were induced to differentiate into adipocytes, which were exposed to EA with different concentrations for 72 h, 0 μmol/L as control group, 1, 5 and 25 μmol/L as exposure groups. Real time-PCR was used for mRNA detection of adipokines （lepin, adiponectin and resistin） mRNA expression. Results Compared with the control group, no significant difference was seen in leptin mRNA expression levels （P〉0.05） in EA exposure groups, the adiponectin mRNA expression levels increased significantly in the condition that human adipocytes were exposed to 1, 5, 25 μmol/L of EA （P〈0.05）, resistin mRNA expression level decreased significantly in the condition that human adipocytes were exposed to 25 μmol/L of EA （P〈0.05）. Conclusion EA may up-regulate the expression of adiponectin mRNA and down-regulate the expression of resistin mRNA.

关 键 词：反油酸 人脂肪细胞因子 瘦素 脂联素 抵抗素 

分 类 号：R994.6[医药卫生—毒理学]