苯并咪唑对雄性大鼠生育力的影响  

Effect of benzimidazole on fertility in male rats

作　　者：于功昌[1] 王筱芬[1] 谢琳[1] 刘衍忠[1] 李莉[1] 

机构地区：[1]山东省医学科学院山东省职业卫生与职业病防治研究院毒理室,山东济南250002

出　　处：《环境与健康杂志》2012年第9期795-798,F0003,共5页Journal of Environment and Health

基　　金：山东省自然科学基金(ZR2011HL037)

摘　　要：目的探讨苯并咪唑对雄性大鼠生育力的影响及其作用靶点。方法将100只健康清洁级Wistar雄性大鼠随机分为4组,分别为低(20 mg/kg)、中(100 mg/kg)、高剂量(200 mg/kg)苯并咪唑染毒组和对照(0.5%吐温80溶液)组,每组25只。采用经口灌胃染毒方式进行染毒,染毒容量为10 ml/kg,每天1次,连续染毒80 d。染毒结束后,雌、雄大鼠以1∶1合笼,观察雌性大鼠妊娠情况,并计算雄性大鼠的交配率和生育力指数。测定精子活动率和精子数量及血清中促黄体生成素(LH)、卵泡刺激素(FSH)和睾酮(T)水平,采用流式细胞学术测定睾丸细胞周期的情况。结果与对照组比较,中、高剂量苯并咪唑染毒组雄性大鼠的交配率和G2期/M期的比例显著升高,生育力指数、精子活动率和精子数量以及睾丸细胞G0期/G1期及S期的比例显著降低,差异均有统计学意义(P<0.05,P<0.01);且随着苯并咪唑染毒剂量的升高,雄性大鼠的交配率和G2期/M期的比例均呈上升趋势,生育力指数、精子活动率和精子数量以及睾丸细胞G0期/G1期及S期的比例均呈下降趋势。与对照组比较,仅高剂量苯并咪唑染毒组雄性大鼠血清中LH水平显著降低,差异有统计学意义(P<0.05),且雄性大鼠血清中LH水平随着苯并咪唑染毒剂量的升高而下降;而各剂量苯并咪唑染毒组雄性大鼠血清中FSH、T水平均无显著变化。结论苯并咪唑对雄性大鼠具有明显的生殖毒性,可引起精子生成障碍,导致雄性大鼠生育力降低,甚至不育。Objective To study the effect of benzimidazole on male rats fertility and its effect target. Methods A total of 100 healthy male Wistar rats were randomly divided into four groups. The rats in low-dose, moderate-dose and high-dose groups were treated with benzimidazole by gavage at the doses of 20, 100 and 200 mg/kg once a day for 80 consecutive days ,while 0.5% tween 80 solution was given to the control. After treatment, the male rats were mated with females at a ratio of 1 ：1 for five days, and the successful mating rate and the fertility index were observed. After mating, the testis and epididymidis were immediately excised. Then the bilateral testes were weighed, and the testis coefficient was calculated. The sperm motility and counts of the left cauda epididymidis were determined. The levels of serum hormone （LH, FSH, T）, histopathological changes and the cell cycle of testis were detected by radio-immunity method, HE staining and flow cytometry, respectively. Results In comparison with control group, the successful mating rates were increased and the fertility indexes were decreased in high-dose and moderate-dose groups exposed to benzimidazole （P〈0.05, P〈0.01）. In high-dose and moderate-dose groups, atrophic testis, decreased testis coefficient, and decreased sperm motility and counts were observed （P〈0.01）. There were no obvious differences in the levels of FSH and T between treatment and control groups. The level of serum LH in high-dose group was lower than that in control group （P〈0.05）. Atrophic seminiferous tubules, disordered and decreased germ cells, increased degeneration, edema and sloughing of germ cells were observed. The ceils in G0/GI, S phases decreased, the accounts of cells in GdM increased with the increase of benzimidazole dose （P〈0.05, P〈0.01 ）. Conclusion Benzimidazole has adverse effect on spermatogenic function in male rats, may cause spermatogenic failure, resulting in decreased fertility or infertility.

关 键 词：苯并咪唑 睾丸 生育力 

分 类 号：R994.6[医药卫生—毒理学]