阳春砂辐射诱变及基于ISSR的变异分析  

Radiation Mutagenesis of Amomum villosum Lour. and its Mutation Analysis Based on ISSR

作　　者：杨锦芬[1] 刘卉[1] 彭苑霞[1] 刘艳[1] 邹伟权 詹若挺[1] 徐晖[1] 陈蔚文[1] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心/教育部(省部共建)中药资源科学重点实验室,广州510006 [2]广东省辐照技术开发中心,广州510642

出　　处：《植物遗传资源学报》2012年第5期748-753,共6页Journal of Plant Genetic Resources

基　　金：国家"十一五"科技支撑计划项目(2006BAI09B01-06);广东省高等学校学科建设专项资金科研类项目(粤教科函[2008]3号)

摘　　要：用组织培养结合辐射诱变的方法进行药用植物阳春砂的育种,对于加快其育种速度、实现高产优质具有重要意义。本研究通过不同浓度的6-BA和NAA配比筛选阳春砂组织培养不定芽的最适培养基,再以组培不定芽为材料,设置不同辐射剂量,进行60Co-γ射线辐射诱变;采用ISSR分子标记技术,对部分诱变材料进行遗传变异分析。结果表明,添加4.0mg/L 6-BA和0.1mg/L NAA的MS培养基不定芽诱导率和出芽倍数最高,分别达到91.4%和2.44;用于阳春砂组培不定芽γ射线辐射的合适剂量为16Gy;ISSR分析结果显示,16Gy辐照获得的2个植株AV16-1和AV16-2在遗传上发生了明显变异,与对照的遗传相似系数分别为0.549和0.563。本研究建立了组织培养结合辐射诱变培育阳春砂新种质的方法,为基于ISSR分子标记技术的诱变育种提供了参考。The combinations of different concentrations 6-BA and NAA were used to screen appropriate culture media for adventitious bud induction of Amomum villosum Lour.The induced buds were radiated by 60Co-γ ray with different irradiation doses and ISSR molecular marker was used to analyze the genetic diversity of the mutants.The optimum culture medium for adventitious buds induction was MS＋6-BA 4.0mg/L＋NAA 0.1mg/L,in which the maximum bud induction rate of 91.4% and multiplication coefficient of 2.44 was attained.The appropriate dose of γ-ray for buds was 16Gy.Two plants irradiated with 16Gy dose,AV16-1 and AV16-2,were mutated according to the genetic similarity coefficients,which were 0.549 and 0.563,respectively.The breeding method of A.villosum by combining tissue culture and radiation mutagenesis to generate new germ plasm was established in this research.

关 键 词：阳春砂 组织培养 Γ射线 辐射诱变 ISSR 

分 类 号：S567.239[农业科学—中草药栽培]