表没食子儿茶素没食子酸酯全乙酰化衍生物的体内抑瘤作用研究  

The Reversal Activity of AcEGCG in vivo

作　　者：欧冰凝[1] 周焕第[2] 梁钢[1] 孙悦文[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西中医学院.药学院,广西南宁530001

出　　处：《时珍国医国药》2012年第9期2116-2118,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30760310);广西壮族自治区自然科学基金(No.2010GXNSFD013043)

摘　　要：目的初步了解表没食子儿茶素没食子酸酯全乙酰化衍生物(AcEGCG)在体内的抑瘤作用。方法建立移植瘤模型,接种BEL-7402/5-FU的裸小鼠随机分为:对照组、5-FU组、AcEGCG组(AE组)、AcEGCG联合5-FU组(AEF组)和EGCG联合5-FU组(EF组)。接种14 d后开始给药,隔日称重、测量肿瘤的大小至给药20 d结束。处死老鼠,取瘤组织,称瘤块重量,计算抑瘤率,绘制肿瘤生长曲线。免疫组化法观察瘤块的病理切片。结果 AEF组的抑瘤率为40.53%,与EF组(30.87%)相比,具有显著性差异(P<0.05)。与5-FU组相比,AEF组裸鼠体重增加,具有显著性差异(P<0.05)。而EF组裸鼠与5-FU组相比减重较少,具有显著性差异(P<0.05)。与EF组相比,AEF组裸鼠体重变化具有显著性差异(P<0.05)。结论 AcEGCG联合5-FU的抑瘤作用比EGCG联合5-FU的增强,且毒性降低。Objective To investigate the reversal activity of AcEGCG in vivo.Methods The models of xenografted tumor in nude mice were established with BEL-7402 /5-FU and received the desired drugs and dosages for 20 days.The animals were randomly separated into five groups,including control group,5-FU group,AE group,AEF group and EF group.The nude mice were weighed and measured tumor volume every two days until the 20th of administration.The nude mice were taken out of tumors with dislocation in cervical spin to their deaths after the end of administration.Results AEF group could increase the inhibiting rate of tumor from 30.87%（EF group） to 40.53%（P 〈 0.05）.Also AcEGCG could effectively lessen weight losing of nude mice because of the toxic of 5-FU.Conclusion AcEGCG is more effective than EGCG in inhibiting tumor of BEL-7402 /5-Fnografts.AcEGCG can lessen the toxic of 5-FU more effectively than EGCG.

关 键 词：AcEGCG 体内 抑瘤作用 

分 类 号：R285.5[医药卫生—中药学]