养阴通脑颗粒中黄芪甲苷含量的HPLC-ELSD测定  

HPLC-ELSD Determination of Astragaloside in Yangyin Tongnao Granules

作　　者：沈滨[1] 万海同[1] 张宇燕[1] 朱振洪[1] 韩进[1] 葛立军[1] 

机构地区：[1]浙江中医药大学生物工程学院,浙江杭州310053

出　　处：《时珍国医国药》2012年第9期2142-2143,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30672652)

摘　　要：目的确定养阴通脑颗粒中黄芪甲苷含量HPLC测定方法及最佳提取工艺。方法用HPLC-ELSD法测定黄芪甲苷含量,色谱柱为Kromasil C18;乙腈-水(34∶66),流速1.0 ml/min,漂移管温度100℃,载气流速为2.0 L/min。采用微波辅助提取,通过正交设计考察溶媒用量、微波功率、微波时间,乙醇体积百分数对黄芪甲苷得率的影响。结果方法学考察结果表明,在0.28～4.20μg(r=0.999 4)范围内,黄芪甲苷呈良好的线性关系;精密度RSD为0.35%;加样回收率为97.43%,RSD为2.59%。最佳提取条件为:微波功率70 W,溶媒用量6倍,微波时间20 min,乙醇体积百分数60%。结论该方法测定结果准确稳定,精密度高,最佳提取工艺下黄芪甲苷的得率高。Objective To determine the best conditions of HPLC - ELSD determination astragaloside and the optimum microwave extraction. Methods An HPLC-ELSD method was set up, using Kromasil Cl8, acetonitrile-water （34：66, v/v）, flow rate was 1.0 ml/min, drift tube temperature 100 ℃, and gas flow rate of 2.0 L/min. An orthogonal experimental design of microwave - assisted extraction was used to study the yield astragalosides by the amount of solvent, microwave power, microwave time, vol- ume percentage of ethanol as the experimental factor. Results The astragaloside showed good linear relationship within a range of 0.28-4.20 μg（r =0. 999 4）. Precision （RSD） was 0.35% ,recovery was 97.43% ,RSD was 2.59%. The optimum conditions were microwave power of 70 W, the amount of 6 times the solvent, microwave time to 20 min, the volume percentage of 60%. Conclusion This method is accurate, stable, highly precised, and with high rate.

关 键 词：养阴通脑颗粒 黄芪甲苷 正交试验 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]