异土木香内酯的微生物转化研究  

Microbial Transformation Research of Isoalantolactone

作　　者：张嫚丽[1] 高洁 王于方[1] 霍长虹[1] 李力更[1] 史清文[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017 [2]石药集团中诺药业有限公司研发一部,河北石家庄050091

出　　处：《时珍国医国药》2012年第9期2171-2173,共3页Lishizhen Medicine and Materia Medica Research

基　　金：河北省自然科学基金(No.08B032;C2010000489);河北省卫生厅科研基金(No.08053);河北医科大学教育科学研究重点项目(No.09zd-17)

摘　　要：目的对异土木香内酯进行微生物转化研究。方法从47株真菌中筛选出对异土木香内酯转化能力强的菌株进行转化。采用色谱法及波谱技术对转化产物进行分离和结构鉴定。结果得到3个转化产物,分别鉴定为:3α-羟基异土木香内酯(1),3α-羟基-11,13-二氢异土木香内酯(2)和2α-羟基异土木香内酯(3)。结论化合物1～3为首次通过微生物转化得到的产物。Objective To investigate the macrobial transformation of isoalantolactone. Methods Forty - seven strains of fungi were screened for their capabilities to transform isoalantolactone. The biotransformation products were isolated by silica gel column chromatography and their structures were elucidated according to the spectral data. Results Three biotranformation products were obtained and identified as 3 - hydroxy - isoalantolactone （ 1 ） ,3 - hydroxy - 11,13-dihydroisoalantolactone （ 2 ） and 2 - hydroxy - isoalantolactone （ 3 ） on the basis of their spectral data. Conclusion Compounds 1 - 3 were obtained through biotransformation of isoalantolactone by fungi for the first time.

关 键 词：异土木香内酯 微生物转化 刺囊毛霉 黑曲霉 

分 类 号：R284.1[医药卫生—中药学]