复方红景天制剂对记忆损伤小鼠的改善作用及机制  

The improving effect and mechanisms of Rhodiola compound on memory-damaged mice

作　　者：张术[1] 曾智发[2] 储智勇[1] 汪家春[1] 

机构地区：[1]海军医学研究所,上海200433 [2]福建中医药大学

出　　处：《中华行为医学与脑科学杂志》2012年第9期797-799,共3页Chinese Journal of Behavioral Medicine and Brain Science

摘　　要：目的观察复方红景天制剂（RCE）对记忆损伤小鼠学习记忆的恢复及生物学机制，为该药的临床应用提供实验依据。方法将小鼠分为复方红景天制剂低、中、高（0．3g／kg，0．6g／kg，1．2∥kg）剂量组和空白对照组，观察给药后小鼠游泳能力；小鼠给药复方红景天制剂（1．2g／kg），利用Morris水迷宫方法测试药物对小鼠东莨菪碱和乙醇记忆损伤的影响。实验结束后小鼠立即处死取脑，匀浆后测定小鼠大脑内超氧化物歧化酶（SOD）活性和一氧化氮（NO）含量。结果复方红景天制剂3个剂量组均能明显延长小鼠力竭游泳时间（P〈0．05，P〈0．01）；高剂量RCE处理后小鼠上平台前游泳距离较模型组显著缩短[乙醇模型组（26906．6±2769．7）mill，RCE处理组（19586．1±6826．7）mm；P〈0．05]，跨平台象限游泳路程和时间均较模型组显著增多（P〈0．05）；RCE组小鼠海马SOD活性较模型组明显增强[东莨菪碱模型组（150．3±17．7）U／ml，RCE处理组（197．9±16．8）U／ml；P〈0．05]，NO含量也明显增加[东莨菪碱模型组（44．7±16．7）μmol／gprot，RCE处理组（65．4±14．5）μmol／gprot；P〈0．05]。结论复方红景天制剂能促进小鼠损伤记忆的恢复，脑内的SOD和NO参与了此改善作用。Objective To investigated the effects of Rhodiola Compound on improving the intellective function in mice and provide the basis for clinical application. Methods Mice were divided to different groups of three doses of rhodiola compound （0.3 g/kg,0.6 g/kg, 1.2 g/kg） and swimming abilities were tested. Other mice were administrated single dose of compound rhodiola（ 1.2 g/kg） and training by Morris water maze. Drug＇s improving intelligence function was assessed using memory acquisition impaired models made by scopolamine or alcohol. When the Morris water maze test was finished, mice were killed and brains were removed immediately to measure SOD and NO levels. Results Groups of three doses of compound rhodiola could significantly prolong the swimming time（P 〈 0.05, P 〈 0.01 ）. Compound Rhodiola group can significantly reduce the swimming distance than the untreated group（ethanol model group： （26 906.6：1：2769.7） mm,RCE treated group： （19 586.1 ：t：6826.7） ram; P〈 0.05 ）. Swimming distance and time of cross-platform quadrant was significantly increased, comparing with model group （P 〈 0.05 ）. Compound Rhodiola significantly enhanced the activity of mouse brain＇s SOD （ Scopolamine model group ： （ 150.3± 17.7 ） U/ml, RCE treated group ： （ 197.9±16.8 ） U/ml ; P 〈 0.05 ） and NO levels （ Scopolamine model group： （44.7 ± 16.7） Ixmo//gprot, RCE treated group： （65.4 ±14.5） p, moL/gprot ; P〈0.05） significantly. Conclusion Compound Rhodiola could promote mice learning and memory function, SOD and NO in brain maybe play a important role in this effect.

关 键 词：复方红景天 学习记忆 小鼠 

分 类 号：R285.5[医药卫生—中药学]