双光子敏化Eu^(3+)高效发光活细胞成像纳米生物探针(英文)  

Nanoprobes with Enhanced Two-Photon-Sensitized Eu～(3+) Luminescence Properties for Live Cell Imaging

作　　者：符小艺[1,2] 邵光胜 韩荣成 马严 薛富民 杨帆[3] 付立民[3] 张建平[3] 王远[1] 

机构地区：[1]北京分子科学国家实验室,分子动态与稳态国家重点实验室,北京大学化学与分子工程学院,北京100871 [2]华南理工大学材料科学与工程学院,广州510640 [3]中国人民大学化学系,北京100872

出　　处：《物理化学学报》2012年第10期2480-2486,共7页Acta Physico-Chimica Sinica

基　　金：supported by the National Natural Science Foundation of China(21073002,21133001,21227803,51121091);National Key Basic Research Special Foundation of China(2011CB808702)~~

摘　　要：将Eu(tta)3dpbt(dpbt:2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine;tta:thenoyltrifluoroacetonato)包埋在甲基丙烯酸甲酯-苯乙烯共聚物、正辛基三甲氧基硅及其水解缩合产物组成的杂化基质中,制备了Eu(tta)3dpbt质量分数为40%的荧光纳米粒子,其平均粒径为45nm.所制备的发光纳米粒子在水中分散稳定性高、光稳定性好、细胞毒性低、长波敏化Eu3+发光性能优良,适宜作为生物分析的发光标记物.所制备的发光纳米粒子的可见区激发峰位于415nm,激发峰尾部延展至475nm,其发光量子产率为0.31(λex=415nm,T=23°C),最大双光子激发作用截面为5.0×105GM(λex=830nm,1GM=10-50cm4·s·photo-1×particle-1).以转铁蛋白修饰上述发光纳米粒子表面制备的纳米生物探针被成功应用于活的HeLa肿瘤细胞的特异性标记和双光子激发Eu3+发光成像.Luminescent nanospheres （EuPHS,d av =45nm） containing 40% （w） of Eu（tta）3 dpbt （tta= thenoyltrifluoroacetonato;dpbt=2-（N,N-diethylanilin-4-yl）-4,6-bis（3,5-dimethylpyrazol-1-yl）-1,3,5-triazine） were prepared by encapsulating Eu（tta）3 dpbt in a hybrid matrix formed in situ from poly（styrene-co-methyl methacrylate）,octyltrimethoxysilane,and poly（octylsiloxane）.The EuPHS are promising luminescent markers for bioanalysis because of their good dispersibility in aqueous solutions,high photostability,low cytotoxicity,and bright Eu3＋ luminescence under excitation at long wavelengths.EuPHS exhibited excellent visible light-sensitized and near-infrared two-photon-sensitized Eu3＋ luminescence properties,with a visible-light excitation peak at 415nm and an excitation window extending up to 475nm.The quantum yield for Eu3＋luminescence was 0.31（λex =415nm,T=23℃）,and the two-photon excitation （TPE） action cross section was 5.0×105GM （1GM=10-50cm4·s· photon-1·particle-1） at 830nm.Bionanoprobes prepared by adsorbing transferrin on the surfaces of EuPHS were successfully applied in target-specific labeling and two-photon-excitation imaging of live HeLa cells.

关 键 词：发光 铕配合物 双光子激发 纳米生物探针 细胞成像 

分 类 号：Q25-3[生物学—细胞生物学] TB383.1[一般工业技术—材料科学与工程]