海湾扇贝与紫扇贝生殖隔离研究  

Reproductive isolation of inter-specific crosses between Argopecten irradians and Argopecten purpuratus

作　　者：张守都[1,2] 张国范[1] 李莉[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《海洋科学》2012年第8期9-14,共6页Marine Sciences

基　　金：国家"863"项目(2006AA10A408;2010AA10A401);国家公益性行业(农业)科研专项(3-35)

摘　　要：对海湾扇贝(Argopecten irradians)和秘鲁紫扇贝(Argopecten purpuratus)进行了跨种间的杂交尝试,结果显示两个物种之间正向和反向均可受精和正常发育,没有发现合子前隔离,并且通过internaltranscribed spacer 1(ITS1)标记验证了杂交的成功。至杂交子一代性成熟时通过对杂合子成熟性腺进行H.E.染色石蜡切片观察发现,雄性性腺明显退化且不能产生有活力的精子,雌性性腺发达且能形成外观正常卵子,但是却不能与亲本进行回交,即表现为典型的合子后隔离:杂种不育。Inter-specific crosses were conducted between Argopecten irradians and Argopecten purpuratus. Bidi- rectional fertilizations and successful hatching were examined for all crosses and no prezygotic isolation was ob- served. The success of hybridization was confirmed by ITS 1 maker. Through the observation of H. E. staining par- affin slice of the gonad of mature hybrids , male gonad was found obsolescence without any active sperm while female gonad was developed with abundant normal appearance eggs. But the hybrids eggs could not back-cross with the parental species, exhibiting typical postzygotic isolation--hybrid sterile.

关 键 词：海湾扇贝(Argopecten irradians) 秘鲁紫扇贝(Argopecten purpuratus) 种间杂交:ITS1 合子后隔离 

分 类 号：S917.3[农业科学—水产科学]