辣椒质膜水通道蛋白CaPIP1基因全长cDNA的分离及序列分析  

Isolation and Sequence Analysis of a Full-Length cDNA Clone Encoding Aquaporin Gene CaPIP1 in Pepper

作　　者：官德义[1] 林金辉[2] 陈成聪[2] 牟少亮[2] 何水林[1,2] 

机构地区：[1]福建农林大学作物科学学院,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《江西农业大学学报》2012年第4期671-675,681,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30600391;30971718);福建省自然科学基金项目(2008J0049)

摘　　要：通过对辣椒均一化cDNA文库的筛选分离获得了一个与烟草水通道蛋白NtAQP1高度同源的aquaporin基因全长cDNA,命名为CaPIP1。序列分析结果表明该cDNA包含有858 bp的完整开放阅读框,编码286个氨基酸,具有6个跨膜区,2个NPA模序以及植物质膜水通道蛋白高度保守的序列。氨基酸同源性及进化分析同样表明CaPIP1为辣椒水通道蛋白家族新成员。One 858 bp full-length eDNA clone was isolated from a pepper normalized cDNA library, which encodes a putative protein composed of 286 amino acids. The full-length eDNA was named CaPIP1. Amino acid sequence deduced by CaPIP1 eDNA showed high similarity to NtAQP1 protein from tobacco. CaPIP1 protein exhibited six transmembrane domains, possessing 2 NPA-motifs and highly conserved sequences of plant aquaporin. Amino acid similarity and phylogenetic analysis also indicated that CaPIP1 was a new member of pepper aquaporin protein superfamily.

关 键 词：辣椒 水通道蛋白 基因克隆 序列分析 

分 类 号：Q78[生物学—分子生物学] S641.3[农业科学—蔬菜学]