杨梅优选单株‘苏杨2号’的生物学特性及其ISSR鉴定  

Biological Characteristics and ISSR Identification of 'SY-2' Waxberry

作　　者：戴斌[1] 徐春明[2] 王利芬[1] 张海如 叶利发 陆小平[1] 

机构地区：[1]苏州大学园艺系,江苏苏州215123 [2]江苏省苏州市林业站,江苏苏州215128 [3]江苏省苏州市吴中区金庭镇农林服务中心,江苏苏州215111 [4]江苏省苏州市吴中区东山镇农林服务站,江苏苏州215107

出　　处：《江西农业大学学报》2012年第4期676-681,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家公益行业(农业)科研专项(201203089);苏州市应用基础研究计划(SYN201012)

摘　　要：从苏州洞庭山的杨梅(Myrica rubra Sieb.et Zucc.)种质资源中筛选到一个优良单株—‘苏杨2号’(SY-2),并对该单株的生物学特性、果实品质及亲缘关系进行分析。运用ISSR-PCR分析方法对10个主栽品种和优选单株‘苏杨2号’进行分析,12个引物,共扩增出90个DNA片段,其中多态性片段70个,占总扩增片段的77.8%;并运用NTSYS-pc 2.10e软件,采用UPGMA法构建了遗传分子系统树。‘苏杨2号’树势强健,树形高大,果实圆球形,果实成熟时紫红色,平均单果重12.63 g,可溶性固形物11.46%,可溶性总糖含量为9.62%,花色苷含量为203.85 nmol/g,6月上中旬成熟,较‘小叶细蒂’早熟5～7 d。ISSR分析表明,在遗传相似系数0.74结合线处,供试杨梅品种可以分为5组,其中‘苏杨2号’与细蒂品种划分在同一组,且‘苏杨2号’与‘小叶细蒂’品种的相似系数最高,为0.777 8。‘苏杨2号’为一早熟、优质的杨梅单株,是一个不同于其它杨梅品种的新品种。To describe the biological characteristics and fruit quality of the excellent individual ＇ SY-2＇ waxberry （Myrica rubra Sieb. et Zucc. ） which was screened from Dongting Mountain in Suzhou, and to determine the genetic relationship with other cuhivars. 10 cuhivars and 1 selected waxberry plant were used in intersimple sequence repeat （ISSR） , total 90 DNA bands were amplified by 12 primers, 70 of which were poly-morphic （77.8%）. A dendrogram was constructed by using UPGMA method, according to ISSR result, by NTSYS-pc 2.10e. ＇ SY-2＇ is of strong growth and tall shape, the fruit is round in shape with purple color, and the mass is 12.63 g on average, containing total soluble solids of 11.46%, SSC of 9.62%, anthocyanin of 203.85 nmol/g, the fruit ripens in early-mid June, 5 to 7 d earlier than ＇ Xiaoyexidi＇. ISSR analysis showed that all cuhivars tested could be classified into 5 groups at 0.74, ＇ SY-2 ＇ and ＇ Xidi＇ are in the same group, and has a close relationship with ＇ Xiaoyexidi＇ , and the similarity coefficient is O. 777 8. ＇ SY-2＇ is a new local variety which is different from other cuhivars, the fruit ripens earlier and is of fine quality.

关 键 词：杨梅 新品种 ISSR 亲缘关系 品种鉴定 

分 类 号：S667.6[农业科学—果树学]