四倍体刺槐插条不定根发生的营养物质变化  

Changes of Nutrients in Adventitious Root Formation of Tetraploid Robinia pseudoacacia Cuttings

作　　者：王小玲[1] 赵忠[2] 高柱[1] 

机构地区：[1]江西省科学院生物资源研究所,江西南昌330029 [2]西北农林科技大学西部环境与生态教育部重点实验室,陕西杨凌712100

出　　处：《江西农业大学学报》2012年第4期724-729,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30972352);江西省科学院省级重点实验室开放基金资助

摘　　要：取四倍体刺槐3年生采穗圃中1年生硬枝和半木质化嫩枝枝条为试材,研究硬枝和嫩枝插条不定根发生过程中可溶性蛋白、可溶性糖、淀粉和植物总氮含量及C/N比值的变化规律,为四倍体刺槐扦插生根的化学调控提供参考依据。结果表明:(1)不定根形成过程中,对照插穗可溶性蛋白含量持续下降;经IBA处理的插穗可溶性蛋白含量先降低后升高,且经IBA处理的硬枝插穗可溶性蛋白含量显著高于对照。(2)插穗可溶性糖含量先升高后降低;经IBA处理的插穗可溶性糖含量明显高于对照,且可溶性糖含量达到高峰的时间较对照提前5 d。(3)经IBA处理的插穗淀粉含量下降的速度大于对照,且经IBA处理的硬枝插穗和对照之间差异水平显著。(4)植物总氮对四倍体刺槐扦插生根的影响作用较小。(5)四倍体刺槐扦插,插穗C/N比值越高,生根能力越强。Annual hardwood and softwood cuttings in 3-year-old cutting orchard of tetraploid Robinia pseudoacacia were taken as materials in this experiment. The c content, starch content, total nitrogen content and C/N ratio hanges of soluble protein content, soluble sugar were studied to provide reference for chemical control of the cuttings of tetraploid Robinia pseudoacacia. The result showed that ： （ 1 ） the soluble protein content of control cuttings kept declining during rooting process. The soluble protein content of IBA-treated cut- tings decreased firstly, and then increased. The soluble protein content of hardwood cuttings pretreated with IBA was significant higher than that of the control. （2） However, the soluble sugar contents of both hardwood and softwood cuttings showed a trend of increasing firstly, and then decreasing. The soluble sugar content of IBA-treated cuttings was higher than that of the control, but showed no significant difference. The highest soluble sugar content of IBA-treated cuttings appeared five days earier than that of the control. （3）The IBAtreated cuttings had a larger speed to degrade starch than the control. At the same time, the cuttings pretreated with IBA showed significant difference. （4） There was little relationship between total nitrogen and the rooting of the cuttings. （5） A positive relationship was detected between C/N ratio and the rooting percentage of tetraploid Robinia pseudoacacia cuttings.

关 键 词：四倍体刺槐 硬枝插穗 嫩枝插穗 营养物质 

分 类 号：S792.27[农业科学—林木遗传育种]