猪痘病毒PCR检测方法的建立及应用  

Development and Application of a PCR Assay for Detection of Swinepox Virus

作　　者：张文波[1] 蒋新华[1] 冷闯[1] 邓舜洲[1] 

机构地区：[1]江西农业大学动物科学技术学院,江西南昌330045

出　　处：《江西农业大学学报》2012年第4期781-785,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31160498/C1803)

摘　　要：建立猪痘病毒(swinepox virus,SWPV)的PCR检测方法,为SWPV的流行病学调查和临床诊断提供可靠技术支撑。参考GenBank登录的SWPV(AF410153.1)基因组序列,设计合成1对引物,扩增目的片段为975 bp。以SWPV江西分离株SWPV-JX01为模板,对反应条件进行优化,建立了SWPV的PCR诊断方法。特异性、敏感性和重复性试验表明,该方法对临床上常见的其它猪病毒检测结果均为阴性;对模板的最低检测量为2.7 pg;具有良好的重复性。用此方法检测50份疑似猪痘的临床样品,其中阳性率为80.2%。该方法敏感度高,重复性和特异性良好,可用于猪痘病毒的分子流行病学调查和临床病例的诊断。A detection method for Swinepox Virus（SWPV） with PCR technique was established as a reliable technological means for epidemiological investigation and clinical diagnosis of SWPV. A pair of primers were designed according to the genome of SWPV to develop a PCR assay for detection of SWPV, the length of the product was 975 bp. The SWPV strain isolated from Jiangxi Province（ SWPV -JX01 ） was taken as templates. The PCR detection method for SWPV was finally established under optimal reaction conditions. The tests for the specificity, sensitivity and repeatability of the PCR assay were conducted, the results indicated that all were negative to the optimal examination method on other pig viruses and the necessary dose of templates of the assay was 2.7 pg. 50 clinical samples, which were suspected SWPV syndrome from Jiangxi Province, were analysed by the method, the result showed that 80.2 % samples（41/50） were SWPV positive. The PCR detection method established in this study has the good repeatability, may be used in molecular epidemiologieal characteristics of sensitivity, high specificity and investigation and rapid clinical diagnosis of SWPV.

关 键 词：猪痘病毒 PCR 猪痘 

分 类 号：S852.659.1[农业科学—基础兽医学]