烟草疫霉生防细菌的筛选及鉴定  

Screening and Identification of a Bacterium Antagonistic to Phytophthora parasitica var. nicotianae

作　　者：舒晓玲[1] 吴毅歆[1] 周惠萍[1] 毛自朝[2] 李顺德[3] 何月秋[1,2] 

机构地区：[1]云南农业大学农业生物多样性应用技术国家工程中心,昆明650201 [2]云南农业大学农学与生物技术学院,昆明650201 [3]玉溪市农业局,云南玉溪663000

出　　处：《中国烟草科学》2012年第4期60-64,共5页Chinese Tobacco Science

基　　金：科技部国际科技合作项目(2009DFA32360);昆明市科技局项目(09H130301)

摘　　要：为获得防治烟草黑胫病的生防菌株,通过对峙平板法,从烟田土壤中分离出1株对烟草疫霉具有很好抑菌作用的细菌B40-3。培养3 d后,该菌株对烟草疫霉抑菌带宽度为0.9 cm,抑菌圈半径为2.3 cm,并对其余12种植物病原真菌有很好抑制作用。该菌株在LB液体培养基中,37℃,210 rpm条件下培养40 h,OD值达到最大值。依传统生理生化反应鉴定,B40-3属于枯草芽孢杆菌。A biocontrol bacterial strain B40-3 antagonistic to Phytophthora parasitica var. nicotianae, a pathogen of tobacco black shrank, was isolated from tobacco-growing field by dual culture. The isolate had 0.9 cm ftmgistasis band and 2.3 cm inhibitory radius when it co-cultured with the pathogen for 3 days. B40-3 could inhibit other 13 fungal pathogens as well. In LB broth medium, its OD600 value reached to the maximum at 37 ~C, 210 rpm for 40 hours. B40-3 was identified as Bacillus subtilis by the traditional spore staining and physiological and biochemical reactions,

关 键 词：烟草疫霉 生防细菌 筛选 鉴定 烟草 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]