仔猪轮状病毒感染与乳糖不耐受症的临床检测  

Clinical detection of piglet rotavirus infection and lactose intolerance

作　　者：李崇[1] 苏亚权[1] 谢娟[1] 林昌华[1] 李凤梅[1] 钟诚[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《南方农业学报》2012年第8期1227-1229,共3页Journal of Southern Agriculture

基　　金：广西大学科研基金项目(X022051)

摘　　要：【目的】探讨轮状病毒感染与乳糖不耐受症的临床相关性,为建立适用于规模化养猪场的仔猪轮状病毒感染检测方法提供理论依据。【方法】随机选择腹泻仔猪和健康仔猪各63头,采用醋酸铅—氢氧化铵法和pH试纸法分别检测仔猪粪便的乳糖和pH值,粪便乳糖大于或等于++且pH≤5.5为继发性乳糖不耐受症;然后运用猪轮状病毒诊断试剂检测乳糖不耐受病例的猪轮状病毒感染情况。【结果】与健康仔猪相比,腹泻仔猪粪便乳糖大于或等于++为36例,其中pH≤5.5有23例,即发生乳糖不耐受症的病例23例,占总腹泻仔猪数的36.51%。对23例乳糖不耐受病例进行轮状病毒感染检测,结果发现仔猪继发性乳糖不耐受症与猪感染轮状病毒所引起腹泻的吻合率高达100%。【结论】由猪轮状病毒所引起的仔猪腹泻,多会发生继发性乳糖不耐受症,采用醋酸铅—氢氧化铵法能及时鉴别诊断猪轮状病毒感染,且具有廉价、方便、快捷等优势,适合在基层猪场大规模使用。[Objective]The experiment was conducted to investigate the clinical correlation between piglet diarrhea and lactose intolerance to provide references for detecting piglet rotavirus in large-scale pig farms. [Method ]Sixty-three diarrheic piglets and sixty-three healthy piglets were randomly selected and divided into two groups. Lactose and pH of piglet feces were measured by the use of lead acetate-ammonia hydroxide method and pH test paper, respectively. Feces lactose≥＋＋ and PHi〈5.5 were diagnosed as secondary lactose intolerance. Then rotavirus diagnostic reagent was used in piglets diagnosed as secondary lactose intolerance to detect rotavirus infection. [Result]The results showed that compared to healthy piglets, 36 pigs＇ feces lactose ≥＋＋, of which, 23 piglets＇ feces pH ≤5.5, meaning 23 lactose intolerance piglets accounted for 36.51% of the total number of diarrheic piglets. Moreover, through detection of rotavirus in each piglet of the 23 piglets, it was found that 100% of secondary lactose intolerance in piglets coincided with diarrhea caused by piglet rotavirus. [ Conclusion]Piglet diarrhea caused by rotavirus often triggered secondary lactose intolerance. Using lead acetate and ammonia hydroxide method could conveniently and rapidly detect rotavirus infection with low cost, which is suitable for large yet primitive pig farms.

关 键 词：仔猪 轮状病毒 腹泻 乳糖不耐受症 醋酸铅-氢氧化铵法 

分 类 号：S858.28[农业科学—临床兽医学]