复方银黄微型灌肠剂对腹腔巨噬细胞5-脂氧合酶产物生成的影响  

EFFECT OF YINHUANG MICRO-ENEMA COMPOUND ONTHE SYNTHESIS OF 5-LIPOXYGENASE PRODUCTS IN RATS

作　　者：廖润玲 刘华钢[2] 杨斌[2] 

机构地区：[1]广西桂林市第二人民医院药剂科,桂林541001 [2]广西医科大学药学院,南宁530021

出　　处：《广西医科大学学报》2012年第4期502-504,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.30260124)

摘　　要：目的:研究复方银黄微型灌肠剂(Yinhuang Micro-enema Compound,YHMCE)对5-脂氧合酶(5-LOX)产物生成的影响,以阐明其抗炎作用的分子机制。方法:体外培养大鼠腹腔巨噬细胞(Peritoneal macrophage,PM),采用高效液相色谱法测定白三烯B4(LTB4)和5-羟二十碳四烯酸(5-HETE)含量变化,以反映5-LOX活性变化。结果:在生药浓度2.24～8.96g/L的范围内,YHMCE呈剂量依赖方式抑制大鼠PM LTB4、5-HETE的生物合成,其IC50分别为含生药2.41g/L与8.43g/L。结论:YHMCE通过抑制5-LOX活性,减少LTB4及5-HETE的产生,可能是其发挥抗炎作用的分子作用机制之一。Objective： To study the effect of Yinhuang Micro-enema Compound （YHMCE） on the synthesis of 5-1ipoxygenase products and elucidate its anti-inflammatory mechanisms. Methods： Peritoneal macrophage （PM） obtained from normal rats was cultivated in vitro. The levels of leukotrienes B4 （LTB4） and 5- hydroxy-eicosatetraenoic acid （5-HETE） were detected with reversed phase high-performance liquid chromatography （RP-HPLC） to reflect the activity of 5-1ipoxygenase （5-LOX）. Results： YHMCE between 2.24 g/L and 8.96 g/L could inhibit the synthesis of LTB4 and 5-HETE with dose-dependent manner in rat peritoneal macrophage. The 50 % inhibiting concentration （IC50） was 2.41 g/L and 8.43 g/L ; respectively. Conclusion： The inhibitive effects of YHMCE on the activity of 5-LOX, LTB4 and 5-HETE may account for its anti-inflammatory actions.

关 键 词：复方银黄微型灌肠剂 腹腔巨噬细胞 5-脂氧合酶 LTB4 5-HETE 

分 类 号：R285[医药卫生—中药学]