五种动物粪便纯培养放线菌的多样性及生物活性  

Diversity and bioactivity of culturable actinobacteria from animal feces

作　　者：姜怡[1] 曹艳茹[1,2] 韩力[3] 靳荣线[1] 郑丹[3] 和文祥[2] 李有龙 黄学石[3] 

机构地区：[1]云南大学省微生物研究所,昆明650091 [2]西北农林科技大学资源环境学院,杨陵712100 [3]中国医科大学代谢病及药物开发实验室,沈阳110001 [4]云南野生动物园,昆明650218

出　　处：《微生物学报》2012年第10期1282-1289,共8页Acta Microbiologica Sinica

基　　金：国家自然科学基金(30900002和81072553);国家重大专项(2009ZX09302-003);美国NIH项目(1P41GM086184-01A1)~~

摘　　要：【目的】以资源利用为出发点,研究了五种动物粪便放线菌的多样性及生物活性。【方法】从云南野生动物园采集5种半野生动物的新鲜粪便样品,用5种培养基分离其中的放线菌,将放线菌鉴定到属,测定纯培养放线菌的抗菌活性,抗肿瘤活性等。【结果】结果表明,所试5种动物粪便放线菌的种类非常丰富,组成各不相同,以链霉菌和微球菌占优势,可能存在大量未知放线菌;粪便放线菌的抗菌活性,抗肿瘤活性,酶活性,分解纤维素、角蛋白的活性非常普遍,尤其是具有抗肿瘤活性的菌株比例较大;产生的具有生物活性的次生代谢产物也是多种多样的。【结论】因此,粪便放线菌与土壤,海洋及植物的放线菌一样,是开发药物、农药和其他产品的重要来源之一,应当加强粪便放线菌的研究和保护利用。[Objectives] In order to provide new source for discovering new lead compounds of drugs and other products,the diversity and some bioactivities of culturable actinobacteria in animal feces were studied.[Methods] Five animals’ fecal samples were collected from Yunnan Wild Animal Park.The pure cultures of actinobacteria were isolated from these samples by using 5 different media.The 16S rRNA gene sequences of 119 selected strains were determined;the phylogenetic analysis was carried out;and antimicrobial and anti-tumor activities were determined by using agar diffusion method,tumor cell lines k562and HL60 respectively.[Results] In total 20 genera of actinobacteria from the 5 animals’ feces were identified.Many strains inhibited Bacillus subtilis,Staphylococcus lentus,Mycobacterium tuberculosis,Candida albicans and Aspergillus niger.Some strains presented antitumor activities.Some known secondary metabolites and Sannastatin,a novel macrolactam polyketide glycoside with bioactivities,were isolated and identified.[Conclusion] Fecal actinobacteria are a new potential source for discovering drug lead and other industry products.

关 键 词：放线菌 多样性 生物活性 动物粪便 

分 类 号：S141.4[农业科学—肥料学]