兔持续性牵张脊髓损伤对脑脊液TNF-α的影响  

Changes of tumor necrosis factor-α after spinal cord injury induced by persistent stretch in cerebrospinal fluid of rabbits

作　　者：吴岳[1] 邹国耀[1] 夏计划[1] 张翔[1] 

机构地区：[1]桂林医学院附属医院脊柱骨病外科,广西桂林541004

出　　处：《重庆医学》2012年第27期2807-2809,共3页Chongqing medicine

摘　　要：目的建立兔持续性牵张脊髓损伤动物模型,探讨脑脊液中TNF-α的变化及其意义。方法将24只健康兔子随机分为4组。A组为正常对照组;B组为假手术组,术中仅安装内固定;C、D组分别牵张L1/2小关节突叠合距离的25%、50%长度,安装内固定以维持牵张距离。测定各组术前及术后10min、2h、24h、7d脑脊液中TNF-α的含量;对各组术前、术后8h、1d、3d、7d进行后肢运动功能Tarlov评分。结果 C、D组脑脊液中TNF-α的含量在早期随着牵张距离的增加而升高,术后10min脑脊液中TNF-α的含量分别为(134.95±19.36)、(155.68±10.63)pg/mL,术后2h分别为(158.13±14.67)、(181.50±16.98)pg/mL,与同组术前、术后24h、术后7d及A、B组同时间比较,差异有统计学意义(P<0.05);后肢运动功能的丧失与脊髓牵张程度有相关性,A、B组牵张术后运动功能无变化,C、D组术前运动功能评分为(5.00±0.00)分,术后8h分别为(4.17±0.41)、(3.67±0.52)分,与同组术前、术后1、3、7d及A、B组同时间比较,差异有统计学意义(P<0.05)。结论脊髓损伤早期脑脊液中TNF-α含量的变化可作为评估脊髓损伤程度及后肢运动功能的一个指标。Objective To establish the animal model of persistent tractive spinal cord injury in rabbits and to investigate the changes of tumor necrosis factor-α（TNF-α） in cerebrospinal fluid and its significance. Methods Totally 24 healthy rabbits were randomly divided into 4 groups .. group A（control group） ,group B（sham-operated group,only installing internal fixation） and group C,D（stretching 25% ,50% of transverse diameter of L1/2 articular surface and installing internal fixation. The level of TNF-α in cerebrospinal fluid was detected before operation and at 10 min, 2 h, 24 h, 7d after operation. The hindlimb motor function in all groups was evaluated by using the Tarlov scoring before operation and at 8h,1d,3d,7d after operation. Results The level of TNF- α in the group C and D had obvious difference with the stretch distance increase in early postoperation. The contents of TNF-a in the group C and D were（134.95±19.36）pg/mL and（155.68±10.63） pg/mL at postoperative 10 min,and（158.13=]=14.67）pg/mL and（181.50±16.98） pg/mL at postoperative 2h. Compared with before operation, postoperative 24 h, 7 d in the same group and the same time points in the group A and B,differences showed statistical significance（P〈0.05）. The lose of hindlimb motor function was correlated with the traction degrees of spinal cord. The motor function of hindlimb in the group A and B without traction had no change. The Tarlov scores of the hindlimb motor function in the group C and D were 5.00±0.00 before operation and 4.17±0.41 and 3.67 ±0.52 at postoperative 8 h. Compared with before operation, postoperative 1,3,7 d in the same group and the same time points in the group A and B,differences were statistically significant（P〈0.05,P〈0.01 respectively）. Conclusion The change of the TNF-a level in cerebrospinal fluid in early spinal cord injury could be used a marker to evaluate the degree of spinal cord injury and the hindlimb motor function.

关 键 词：肿瘤坏死因子Α 脊髓损伤 脑脊液 牵张 兔 

分 类 号：R651.2[医药卫生—外科学]