Decorin基因转染对人胃癌裸鼠移植瘤生长的影响  

Effect of decorin gene transfection on human gastric carcinoma xenograft growth in nude mice

作　　者：刘波[1] 姜政[1] 王川[1] 胡在敏[1] 季文[1] 蔡璨[1] 亢渝俊[1] 

机构地区：[1]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《细胞与分子免疫学杂志》2012年第10期1009-1012,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:探讨核心蛋白聚糖(DCN)对人胃癌细胞SGC-7901裸鼠移植瘤生长的影响及可能的机制。方法:构建裸鼠胃癌移植瘤模型,将重组质粒pEGFP-N1-DCN注入肿瘤中央及基底部,以空质粒组和生理盐水组作为对照,测量肿瘤体积和质量的变化,RT-PCR及Western blot法检测DCN的表达,Western blot法检测转化生长因子-β1(TGF-β1)和基质金属蛋白酶-9(MMP-9)的表达,免疫组织化学染色方法检测血管内皮生长因子(VEGF)的表达。结果:裸鼠胃癌移植瘤模型构建成功,与对照组比较,重组质粒组肿瘤生长速度减慢,肿瘤体积和质量明显变小(P<0.05);RT-PCR显示重组质粒组DCN表达增强(P<0.05),Western blot结果显示重组质粒组有融合蛋白的表达,重组质粒组TGF-β1及MMP-9蛋白表达均降低(P<0.05);免疫组化显示重组质粒组VEGF表达降低(P<0.05)。结论:DCN基因可以抑制裸鼠移植瘤生长,其机制可能与转化生长因子-β1及基质金属蛋白酶-9蛋白的表达降低和肿瘤新生血管形成抑制有关。AIM： To investigate the effect of decorin （DCN） gene transfection on the growth of nude mice xenograft bearing human gastric carcinoma cell SGC-7901 and study the possible mechanism. METHODS： Gastric carcinoma xenograft model was established in nude mice. Recombi- nant plasmid pEGFP-N1-DCN was injected to the center and bottom of the carcinoma; meanwhile, the empty plasmid or physiological saline was injected in the other models as controls. We monitored the changes of carcinoma volume and weight, detected the expression of DCN by RT-PCR and Western blotting, the expressions of transforming growth factor-β1 （TGF-β1） and matrix metalloproteinases-9 （MMP-9） by Western blotting, and the expression of vascular endothelial growth factor （VEGF） by immunohisto- chemistry. RESULTS： Gastric carcinoma xenograft models were constructed successfully in nude mice. Compared with the control groups, slower growth speed, smaller volume and lighter weight were found in recombinant plasmid group （P〈 0.05）. RT-PCR showed that the expression of DCN gene in recombinant plasmid group was significantly higher than that in the control groups （ P 〈 0.05） ; Western blotting indicated the expression of fusion protein in recombinant plasmid group; Western blotting also showed that the expressions of TGF-β1 and MMP-9 protein were lower in recombinant plasmid group than in the control groups （ P 〈 0.0,5 ）; immunohistochemistry revealed that the expression of VEGF was down-regulated in recombinant plasmid group （P 〈 0. 05）. CONCLUSION： DCN gene can inhibit the growth of nude mice xenograft, which is probably related with the decreased expressions of TGF-1β and MMP-9protein and the inhibition of tumor angiogenesis.

关 键 词：核心蛋白聚糖 转染 胃癌 转化生长因子-Β1 基质金属蛋白酶-9 血管内皮生长因子 

分 类 号：R392.33[医药卫生—免疫学] R735.2[医药卫生—基础医学]