成年大鼠延髓薄片制备的改良方法  

A modified method of medullary slice preparation in adult rats

作　　者：罗道枢[1] 朱玲 陈涛[3] 黄汾生[3] 王玮[1] 李云庆[3] 

机构地区：[1]福建医科大学解剖与组胚学系,福州350108 [2]兰州军区军医训练大队基础医学教研室,呼图壁831200 [3]第四军医大学人体解剖与组织胚胎学教研室,西安710032

出　　处：《神经解剖学杂志》2012年第5期469-473,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30771133;30971123;31010103909;81200867)

摘　　要：目的:建立一种适用于膜片钳记录研究的成年大鼠延髓薄片制备方法。方法:用改制的注射器将延髓和上颈髓段从离断的椎管中直接吹出;采用水平切的方式制备延髓薄片;记录三叉神经脊束核尾侧亚核(Vc)神经元的自发放电和诱发放电活动。结果:分离得到的延髓和上颈髓标本光滑完整;水平切的延髓薄片较好地保持了Vc的形态学结构和神经元活性,可较好地记录到Vc神经元自发的兴奋性突触后电流和诱发的双脉冲抑制活动。结论:本方法操作简单,取材快速,延髓薄片外形完整且能保持神经元活性,适用于成年大鼠Vc的膜片钳研究。Objective： To establish a suitable method to prepare medullary slice for patch-clamp recording study in adult rats. Methods ： Using a modified syringe to blow the medulla oblongata and upper cervical cord specimen out of the vertebral canal. The medullary slices were prepared by horizontal cutting the specimen. Then spontaneous and evoked dischar- ges of neurons in the caudal subnucleus of the spinal trigeminal nucleus （Vc） were recorded with voltage patch-clamp. Results： The prepared specimen was smooth and neat. The medullary slices with horizontal cutting maintained good morphological structure. The neuronal activities, such as spontaneous excitatory postsynaptic currents and evoked paired- pulse depression in the Vc were also recorded with easy. Conclusion： This method was simple and quick. The morphological features and neuronal activities of the medullary slice were kept without any mechanical damage. It means that the method used in the present study is suitable for patch-clamp recording study in the Vc of the adult animal.

关 键 词：成年大鼠 延髓 薄片 膜片钳 电生理特征 三叉神经脊束核尾侧亚核 

分 类 号：R446.8[医药卫生—诊断学]