他克莫司对人Jurkat淋巴瘤细胞分泌IL-6和sIL-2R及表达IL-6和sIL-2RmRNA的影响  

Effects of tacrolimus on the secretion of interleukin-6 （IL-6）, soluble interleukin-2 receptor （sIL-2） and expression of IL-6 and sIL-2R mRNA by a human lymphoma cell line Jurkat

作　　者：张峻岭[1] 闫翠彦[2] 索丹凤[3] 马秀亮[1] 

机构地区：[1]天津市长征医院皮肤色素科,300120 [2]北京市丰台区妇幼保健院皮肤科 [3]天津市第一中心医院皮肤科

出　　处：《中华皮肤科杂志》2012年第10期745-746,共2页Chinese Journal of Dermatology

基　　金：中华医学会皮肤性病学分会一安斯泰来皮肤病学研究基金

摘　　要：目的探讨他克莫司对淋巴细胞分泌IL-6和sIL-2R及表达1L-6和sIL-2RmRNA的影响。方法酶联免疫吸附试验测定不同浓度他克莫司对人Jurkat淋巴瘤细胞产生IL-6和sIL-2R的影响，实时荧光定量PCR法分析他克莫司对淋巴瘤细胞IL-6mRNA和sIL-2RmRNA表达的影响。结果102-10nmol／L他克莫司可以抑制Jurkat淋巴瘤细胞分泌IL-6、sIL-2R（P值均〈0．05），其中10^3-10^4 nmol／L他克莫司作用显著。10^2nmol／L他克莫司可下调淋巴瘤细胞IL-6和sIL-2RmRNA的表达（P值均〈0．05）。结论适当浓度他克莫司可抑制淋巴细胞分泌IL-6和sIL-2R，下调IL-6和sIL-2RmRNA的表达。Objective To evaluate the effects of tacrolimus on the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes. Methods Jurkat human lymphoma cells were cultured and treated with tacrolimus of different concentrations. Enzyme linked immunosorbent assay was performed to determine the levels of IL-6 and sIL-2R in the supernatant of Jurkat cells at 48 hours after treatment with tacrolimus of 0, 10, 102, 103 and 104 nmol/L, and real time reverse transcription PCR to measure the expression of IL-6 mRNA and sIL-2R mRNA of Jurkat cells at 48 hours after treatment with tacrolimus of 102 nmol/L. Results Tacrolimus of 102 - l04 nmol/L could suppress the secretion of [L-6 and sIL-2R from Jurkat cells （all P 〈 0.05）, with a more marked suppressing effect achieved by the use of tacrolimus at 103 - 104 nmol/L. The expressions of IL-6 and sIL-2R mRNA from Jurkat cells were downregulated by tacrolimus of 102 nmol/L （both P 〈 0.05）. Conclusion Tacrolimus at certain concentrations could downregulate the secretion of IL-6 and slL-2R as well as the expression of IL-6 and slL-2R mRNA by lymphocytes

关 键 词：IL-6MRNA t淋巴瘤细胞 SIL-2R 他克莫司 细胞分泌 实时荧光定量PCR法 酶联免疫吸附试验 mRNA表达 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]