血清白三烯B4水平、ALOX5AP基因SG13S89G/A多态性与急性心肌梗死的关联性研究  

Interrelation among serum LTB4 level,arachidonate 5-lipoxygenase activating protein gene SG13S89G/A polymorphism and acute myocardial infarction

作　　者：叶姗 何国平[1] 惠静姣 沈丹丹 戚传平[2] 许联红[2] 钱亿超[1] 

机构地区：[1]江苏大学附属武进医院心内科,江苏常州213002 [2]江苏大学附属武进医院中心实验室

出　　处：《临床心血管病杂志》2012年第9期674-677,共4页Journal of Clinical Cardiology

基　　金：常州市卫生局重大科研项目(No:ZD200804)

摘　　要：目的:探讨中国常州地区汉族人群血清白三烯(LT)B4水平、花生四烯酸5-脂氧合酶激活蛋白(ALOX5AP)基因SG13S89G/A多态性和急性心肌梗死(AMI)三者的相互关联性。方法:以262例AMI患者(AM I组)和132例非冠心病患者(对照组)作为研究对象,采用聚合酶链反应-限制性片段长度多态性方法检测ALOX5AP基因SG13S89G/A多态性,同时采用酶联免疫吸附试验检测血清LTB4水平(以中位数/四分位数间距表示)。结果:对照组与AMI组ALOX4AP基因SG13S89G/A位点的(AA+GA)基因型(7.58%︰4.96%)、GG基因型(92.42%︰95.04%)以及A等位基因(4.17%︰2.67%)频率均差异无统计学意义;多变量Logistic回归分析显示,该位点多态性与AMI发病风险无显著相关性;AMI组血清LTB4水平显著高于对照组(477.97/370.52pg/ml︰200.57/236.65pg/ml,P<0.01);多变量Logistic回归分析显示,血清LTB4水平与AMI发病风险有显著相关性(P<0.01);AMI组和对照组的组内(AA+GA)基因型和GG基因型之间的血清LTB4水平比较差异无统计学意义。结论:中国常州地区汉族人群AMI患者血清LTB4水平显著升高,ALOX5AP基因SG13S89G/A多态性与AMI易感性无关,且不影响血清LTB4水平。Objective：To investigate the interrelation among the serum leukotriene（LT） B4 level,the arachidonate 5-lipoxygenase activating protein（ALOX5AP） gene SG13S89G/A and the risk of acute myocardial infarction in the Chinese Han population of Changzhou region. Method：The polymorphism in the ALOX5AP gene SG13S89G/A was genotyped by polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP） analysis and the serum LTB4 level was measured by enzyme-linked immunosorbent assay（ELISA） in 262 patients with AMI（AMI group） and 132 subjects（control group） with chest pain but without coronary heart disease by coronarography. Result：Compared to control group,there were no significant differences in AMI group in frequencies of ALOX5AP gene SG13S89G/A locus（AA＋GA） genetype（7.58%︰4.96%）,GG genetype（92.42%︰95.04%） and A allele（4.17%︰2.67%）.Multivariable logistic regression analysis showed that there was no significant diffirence between ALOX5AP gene SG13S89G/A polymorphism and the risk of AMI.The serum LTB4 level（M/IQR） in AMI group was significantly higher than that in control group（477.97/370.52 pg/ml︰200.57/236.65 pg/ml,P〈0.01）.Multivariable logistic regression analysis showed that the serum LTB4 level was significant correlation with the risk of AMI（P〈0.01）.There was no significant difference in serum LTB4 level between GG genetype and（AA＋GA） genetype in both groups. Conclusion：Serum LTB4 level is significantly increased in patients with AMI.ALOX5AP gene SG13S114G/A polymorphism may not be correlated to the risk of AMI and may not influence the serum LTB4 level in Chinese Han population of Changzhou region.

关 键 词：心肌梗死 急性 花生四烯酸5-脂氧合酶激活蛋白 血清LTB4 基因多态性 

分 类 号：R542.2[医药卫生—心血管疾病]