泡球蚴原头蚴抗原和卡介苗对Th1/Th2转录因子和细胞因子的影响  

Effects of Echinococcus spp.natural antigens and BCG on the expression of Th1/Th2 transcriptional factor and cytokines

作　　者：李红卫[1,2] 袁芳[1] 方丽梅[1] 杨玉荣[1] 

机构地区：[1]宁夏医科大学基础学院病原生物学教研室,银川750004 [2]大同市第三人民医院血液内科,037008

出　　处：《免疫学杂志》2012年第10期918-920,共3页Immunological Journal

基　　金：自然科学基金项目(30960339)

摘　　要：目的探讨泡球蚴原头蚴抗原和卡介苗免疫小鼠对泡球蚴攻击感染的调节机制。方法用实时定量聚合酶链反应检测鼠脾组织中GATA-3及T-bet的mRNA表达水平;酶联免疫吸附法检测鼠血清中白介素4和γ干扰素的含量。结果卡介苗免疫攻击组与PBS对照组的转录因子(T-bet mRNA)和其标志性细胞因子(INF-γ)的表达量,差异有统计学意义(P<0.05)。结论实验证明卡介苗(BCG)有上调Th1型免疫反应的作用,用BCG可以干预或治疗由泡球蚴抗原诱导的晚期泡球蚴(AE)动物的免疫抑制状态。We aimed to investigate the cellular immune regulating effects of of Echinococcus spp.natural antigens and BCG in E.multilocularis protoscolex-challenged Kunming mice.We applyed qRT-PCR to detect the quantity of the transcriptional regulators of T-bet and GATA-3 in spleen tissue of experimental mice,while used capture-ELISA to detect the signature Th1/Th2 cytokines of IFN-γ/IL-4 in serum.We found that BCG could up-regulated T-bet mRNA expression and signature cytokine of IFN-γ（P〈0.05）.However,no significant changes of GATA-3 mRNA and IL-4 expressions was found in BCG immunized mice（P〉0.05）.From all the result,we draw that BCG may be used for intervention when immune-suppression is induced in AE sufferance.

关 键 词：卡介苗 泡球蚴原头蚴抗原 T-bet/GATA-3调节通路 Th1/Th2特异性细胞因子 

分 类 号：R392.1[医药卫生—免疫学]