主要精液参数及精子DNA完整率与不明原因习惯性流产的相关性  

Correlation of recurrent pregnancy loss with sperm parameters and sperm DNA fragmentation

作　　者：王英俊[1] 李大文[3] 张唯力[1] 张荣秋[1] 王高能[1] 张融融[2] 

机构地区：[1]重庆医科大学附属第二医院泌尿外科,400010 [2]重庆医科大学2012级研究生 [3]广西壮族自治区人民医院遗传与生殖中心

出　　处：《中华医学遗传学杂志》2012年第5期602-605,共4页Chinese Journal of Medical Genetics

摘　　要：目的研究主要精液参数和精子DNA完整率与习惯性流产的相关性。方法收集配偶具有习惯性流产史的患者85例，其精液标本与正常生育的男性（20名）进行对比研究。精子DNA检测采用精子染色质扩散试验法。结果在精液常规方面，精子活力与习惯性流产存在相关性（P〈0．05），习惯性流产组精子DNA断裂指数为（34．99±14．62）％，对照组为（10．82±4．80）％，两者比较差异具有统计学意义（P〈0．01）。结论精子活力与习惯性流产存在相关性，习惯性流产组患者与正常生育组比较，具有较高精子DNA损伤。Objective To evaluate and compare standard sperm parameters and sperm DNA fragmentation in seminal ejaculates from men whose partners had a history of recurrent pregnancy loss （RPL） and a control group of men who had recently established their fertility. Methods Semen samples frorfi 85 patients with a history of RPL and 20 men with proven fertility were analyzed according to World Health Organization guidelines. Sperm DNA fragmentation was detected by sperm chromatin dispersion test （SCD）. Results A significant difference （P〈0. 05） was observed in sperm motility but not other parameters between the two groups. The mean number of sperm cells with fragmented DNA, represented as DNA fragmentation index, was significantly increased in the RPL group [（34. 99 ± 14. 62）%] compared with controls [（10.82±4.80） %]. Conclusion This study has indicated that sperm from men with a history of RPL have a higher incidence of DNA damage and poor motility compared with fertile males.

关 键 词：精液参数 精子DNA完整率 习惯性流产 精子染色质扩散试验 

分 类 号：R698.2[医药卫生—泌尿科学]