BMP-2对人退变椎间盘髓核细胞影响的实验研究  

Effects of BMP-2 on human nucleus pulposus cells in the disc degeneration

作　　者：梁伟国[1] 叶冬平[1] 李锋生[1] 戴丽冰[1] 沈雁[1] 陈鸿辉[1] 

机构地区：[1]暨南大学第四附属医院广州市红十字会医院,510220

出　　处：《中国矫形外科杂志》2012年第19期1782-1785,共4页Orthopedic Journal of China

基　　金：广州市医药卫生重点项目(编号:2009-zdi-04)资助;2010年广东省自然科学基金(项目编号:10151022001000005);2011年广东省自然科学基金(项目编号:S2011010000910)资助

摘　　要：[目的]探讨BMP-2对人退变髓核细胞合成细胞外基质的影响。[方法]分离、培养人退变椎间盘髓核细胞,取第2代髓核细胞,随机将退变椎间盘髓核细胞分为2组。A组:加入100 ng/ml BMP-2,B组:加入200 ng/mlBMP-2,C组:对照组,不加干扰因素。通过对试验组和对照组髓核细胞采用光镜、电镜等形态学方法进行大体形态和超微结构观察,细胞Ⅱ型胶原和糖胺多糖的mRNA表达。ELISA检测细胞培养上清中人Ⅱ型胶原含量,DMMB比色法检测细胞培养上清中糖胺多糖含量。[结果]髓核细胞中II型胶原、糖胺多糖表达水平实验组均高于对照组。[结论]BMP-2蛋白可促进退变腰椎间盘细胞分泌蛋白多糖和Ⅱ型胶原,增加细胞活性,恢复椎间盘的功能和活性,因此运用BMP-2椎间盘内注射有望成为椎间盘退变疾病生物治疗的方法之一。[ Objective] To determine the effect of BMP- 2 on extracellular matrix and gene expression on the human de- generated nucleus pulposus cells. [Methods] The second passage of the NP cells were divided into 2 groups. They were group A adding 100 ng/mlBMP -2, group B adding 200 ng/mlBMP -2, and group C, control group, without interfering factors. The type II collagen and GAG mRNA expression were test, type II collagen content and glycosaminoglycan content of the supernatant were test. [Results] BMP -2 can stimulate the degenerated nucleus pulposus cells to create the type II collagen, GAG, Sox - 9. [ Conclusion] 100 ng/ml, 200 ng/ml of BMP-2 can extend the degeneration of nucleus pulposus cells in vitro aging, increes the type II collagen, GAG, Sox - 9 mRNA expression levels , reverse degeneration of the intervertebral disc cells to some extent.

关 键 词：髓核细胞 Sox-9 BMP-2 

分 类 号：R681.53[医药卫生—骨科学]